Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings.

ABSTRACT:

Background

Standard nasopharyngeal swab testing for SARS-CoV-2 detection by PCR is not always feasible due to limitations in trained personnel, personal protective equipment, swabs, PCR reagents, and access to cold chain and biosafety hoods.

Methods

We piloted the collection of nasal mid-turbinate swabs amenable to self-testing, including both standard polyester flocked swabs as well as 3D printed plastic lattice swabs, placed into either viral transport media or an RNA stabilization agent. Quantitative SARS-CoV-2 viral detection by RT-qPCR was compared to that obtained by nasopharyngeal sampling as the reference standard. Pooling specimens in the lab versus pooling swabs at the point of collection was also evaluated.

Results

Among 275 participants, flocked nasal swab

SUBMITTER: Muller MS 

PROVIDER: S-EPMC7924294 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature