Ontology highlight
ABSTRACT: Objective
The antiretroviral-based dapivirine vaginal ring reduced HIV risk among women in phase III clinical trials. However, limited data exists on the impact of dapivirine on the vaginal microenvironment in adolescents.Design
A comprehensive metaproteomics approach was used to assess host proteome and microbiome changes in cervicovaginal mucus with dapivirine ring use in adolescents enrolled in the MTN-023/IPM 030 (MTN-023) trial.Methods
Participants were randomized 3?:?1 to use dapivirine or placebo vaginal rings monthly for 6 months. Cervicovaginal samples from a subset of 35 participants (8 placebo, 27 dapivirine) were analyzed.Results
Mass spectrometry analysis identified 405 human and 2467 bacterial proteins belonging to 15 unique genera. The host proteome belonged to many functional pathways primarily related to inflammation. When stratified by study treatment arm, 18 (4.4%) and 28 (6.9%) human proteins were differentially abundant (adjusted P?ConclusionCondom use may associate with differences to inflammation and bacterial function but dapivirine ring use does not, thereby supporting the mucosal safety profile of this vaginal ring for adolescents.
SUBMITTER: Farr Zuend C
PROVIDER: S-EPMC7924934 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Farr Zuend Christina C Noël-Romas Laura L Hoger Sarah S McCorriser Stuart S Westmacott Garrett G Marrazzo Jeanne J Hillier Sharon L SL Dezzutti Charlene C Squires Kathleen K Bunge Katherine E KE Burgener Adam A
AIDS (London, England) 20210301 3
<h4>Objective</h4>The antiretroviral-based dapivirine vaginal ring reduced HIV risk among women in phase III clinical trials. However, limited data exists on the impact of dapivirine on the vaginal microenvironment in adolescents.<h4>Design</h4>A comprehensive metaproteomics approach was used to assess host proteome and microbiome changes in cervicovaginal mucus with dapivirine ring use in adolescents enrolled in the MTN-023/IPM 030 (MTN-023) trial.<h4>Methods</h4>Participants were randomized 3 ...[more]