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The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.

ABSTRACT: The ancestral strain of Bacillus subtilis NCIB3610 (3610) bears a large, low-copy-number plasmid, called pBS32, that was lost during the domestication of laboratory strain derivatives. Selection against pBS32 may have been because it encodes a potent inhibitor of natural genetic competence (ComI), as laboratory strains were selected for high-frequency transformation. Previous studies have shown that pBS32 and its sibling, pLS32 in Bacillus subtilis subsp. natto, encode a replication initiation protein (RepN), a plasmid partitioning system (AlfAB), a biofilm inhibitor (RapP), and an alternative sigma factor (SigN) that can induce plasmid-mediated cell death in response to DNA damage. Here, we review the literature on pBS32/pLS32, the genes found on it, and their associated phenotypes.

SUBMITTER: Burton AT

PROVIDER: S-EPMC7925076 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.

Burton Aisha T AT Kearns Daniel B DB

Journal of bacteriology 20200825 18

The ancestral strain of Bacillus subtilis NCIB3610 (3610) bears a large, low-copy-number plasmid, called pBS32, that was lost during the domestication of laboratory strain derivatives. Selection against pBS32 may have been because it encodes a potent inhibitor of natural genetic competence (ComI), as laboratory strains were selected for high-frequency transformation. Previous studies have shown that pBS32 and its sibling, pLS32 in Bacillus subtilis subsp. natto, encode a rep ...[more]

PMID: 32601071

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