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Morphological and Mechanical Properties of Electrospun Polycaprolactone Scaffolds: Effect of Applied Voltage.

ABSTRACT: The aim of this work is to investigate the effect of the applied voltage on the morphological and mechanical properties of electrospun polycaprolactone (PCL) scaffolds for potential use in tissue engineering. The morphology of the scaffolds was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and the BET techniques for measuring the surface area and pore volume. Stress-strain curves from tensile tests were obtained for estimating the mechanical properties. Additional studies for detecting changes in the chemical structure of the electrospun PCL scaffolds by Fourier transform infrared were performed, while contact angle and X-ray diffraction analysis were realized for determining the wettability and crystallinity, respectively. The SEM, AFM and BET results demonstrate that the electrospun PCL fibers exhibit morphological changes with the applied voltage. By increasing the applied voltage (10 to 25 kV) a significate influence was observed on the fiber diameter, surface roughness, and pore volume. In addition, tensile strength, elongation, and elastic modulus increase with the applied voltage, the crystalline structure of the fibers remains constant, and the surface area and wetting of the scaffolds diminish. The morphological and mechanical properties show a clear correlation with the applied voltage and can be of great relevance for tissue engineering.

SUBMITTER: Can-Herrera LA

PROVIDER: S-EPMC7926916 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Morphological and Mechanical Properties of Electrospun Polycaprolactone Scaffolds: Effect of Applied Voltage.

Can-Herrera L A LA Oliva A I AI Dzul-Cervantes M A A MAA Pacheco-Salazar O F OF Cervantes-Uc J M JM

Polymers 20210223 4

The aim of this work is to investigate the effect of the applied voltage on the morphological and mechanical properties of electrospun polycaprolactone (PCL) scaffolds for potential use in tissue engineering. The morphology of the scaffolds was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and the BET techniques for measuring the surface area and pore volume. Stress-strain curves from tensile tests were obtained for estimating the mechanical properties. Addi ...[more]

PMID: 33672211

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Project description:The development of living heart valves that grow with the patient is a promising strategy for heart valve replacements in pediatric patients. Despite active research in the field of tissue engineered heart valves there have been limited efforts to optimize the balance between biodegradation of the scaffolds and de novo extracellular matrix (ECM) synthesis by cells and study their consequences on the mechanical properties of the cell-seeded construct. This study investigates the effect of in vitro degradation and ECM secretion on the mechanical properties of hybrid polyester scaffolds. The scaffolds were synthesized from blends of fast degrading polyglycerol sebacate (PGS) and slowly degrading polycaprolactone (PCL). PGS-PCL scaffolds were electrospun using a 2:1 ratio of PGS to PCL. Accelerated hydrolytic degradation in 0.1 mM sodium hydroxide revealed 2-fold faster degradation of PGS-PCL scaffolds compared with PCL scaffolds. Thermal analysis and scanning electron microscopy demonstrated marginal change in PCL scaffold properties, while PGS-PCL scaffolds showed preferential mass loss of PGS and thinning of the individual fibers during degradation. Consequently, the mechanical properties of PGS-PCL scaffolds decreased gradually with no significant change for PCL scaffolds during accelerated degradation. Valvular interstitial cells (VICs) seeded on PGS-PCL scaffolds showed higher ECM protein secretion compared with PCL. Thus the mechanical properties of the cell-seeded PCL scaffolds did not change significantly compared with acellular scaffolds, probably due to slower degradation and ECM deposition by VICs. In contrast, the PGS-PCL scaffolds exhibited a gradual decrease in the mechanical properties of the acellular scaffolds due to degradation, which was compensated for by new matrix secreted by VICs seeded on the scaffolds. Our study demonstrated that the faster degrading PGS component of PGS-PCL accelerated the degradation rate of the scaffolds. VICs, on the other hand, were able to remodel the synthetic scaffold, depositing new matrix proteins and maintaining the mechanical properties of the scaffolds.

Dataset Information

Morphological and Mechanical Properties of Electrospun Polycaprolactone Scaffolds: Effect of Applied Voltage.

Publications

Morphological and Mechanical Properties of Electrospun Polycaprolactone Scaffolds: Effect of Applied Voltage.

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