Dataset Information

Recruitment of ?? T cells to the lesion via the CCL2/CCR2 signaling after spinal cord injury.

ABSTRACT:

Background

Immune cell infiltration and neuroinflammation are heavily associated with spinal cord injury (SCI). C-C motif chemokine ligand 2/C-C chemokine receptor type 2 (CCL2/CCR2) axis has been identified as a critical role player during the invasion of immune cells to lesions in many diseases. ?? T cells, a subgroup of T cells, manage the course of inflammation response in various diseases; however, it remains unknown whether ?? T cells are recruited to injury site through CCL2/CCR2 signaling and exert the regulation effect on neuroinflammation after SCI.

Methods

Basso Mouse Scale (BMS), regularity index, cadence, max contact area, and motor-evoked potential testing (MEP) were measured to determine the neurological function recovery after spinal cord injury. Nissl staining was performed to identify the number of surviving motor neurons at lesion epicenter. Immunofluorescence, Western blot, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time polymerase chain reaction (QRT-PCR) also were employed to evaluate the expression of associated proteins and genes.

Results

In this study, we demonstrated that TCR?^-/- mice present improved neurological recovery after SCI. ?? T cell recruitment to the SCI site was significantly reduced and motor functional improvement enhanced in CCL2^-/- and CCR2^-/- mouse strains. Furthermore, reconstitution of TCR?^-/- mice with ?? T cells extracted from CCR2^-/- mice also showed similar results to CCL2 and CCR2 deficient mice.

Conclusions

In conclusion, ?? T cell recruitment to SCI site promotes inflammatory response and exacerbates neurological impairment. CCL2/CCR2 signaling is a vital recruitment mechanism of ?? T cells to the SCI site, and it may be taken as a novel therapeutic target for future SCI.

SUBMITTER: Xu P

PROVIDER: S-EPMC7927238 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Publications

Recruitment of γδ T cells to the lesion via the CCL2/CCR2 signaling after spinal cord injury.

Xu Ping P Zhang Feng F Chang Min-Min MM Zhong Cheng C Sun Cheng-Hong CH Zhu Hao-Ran HR Yao Jing-Chun JC Li Zhi-Zhong ZZ Li Si-Tao ST Zhang Wen-Cai WC Sun Guo-Dong GD

Journal of neuroinflammation 20210302 1

<h4>Background</h4>Immune cell infiltration and neuroinflammation are heavily associated with spinal cord injury (SCI). C-C motif chemokine ligand 2/C-C chemokine receptor type 2 (CCL2/CCR2) axis has been identified as a critical role player during the invasion of immune cells to lesions in many diseases. γδ T cells, a subgroup of T cells, manage the course of inflammation response in various diseases; however, it remains unknown whether γδ T cells are recruited to injury site through CCL2/CCR2 ...[more]

PMID: 33653377

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