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The complete chloroplast genome sequence of Populus szechuanica, a medicinal plant for child anesthesia application.

ABSTRACT: Populus szechuanica C.K. Schneid. is used for wind and sand fixation, farmland protection, soil and water conservation. And some varieties bud fat and inflorescence are available for medicinal use; poplar bark contains tannin and can be used as tanning material. It is also widely used in afforestation and greening in cities across China. In addition, the P. szechuanica also showed vital important application values on the child anesthesia, which could significantly reduce the occurrence of hypotension after anesthesia. The complete chloroplast genome sequence of P. szechuanica was characterized from Illumina pair-end sequencing. The chloroplast genome of P. szechuanica was 156,717 bp in length, containing a large single-copy region (LSC) of 84,900 bp, a small single-copy region (SSC) of 16,527 bp, and two inverted repeat (IR) regions of 27,645 bp. The overall GC content is 36.70%, while the corresponding values of the LSC, SSC, and IR regions are 34.5%, 30.7%, and 41.9%, respectively. The genome contains 131 complete genes, including 86 protein-coding genes (68 protein-coding gene species), 37 tRNA genes (29 tRNA species) and 8 rRNA genes (4 rRNA species). The Maximum Likelihood phylogenetic analysis showed that P. szechuanica and P. koreana clustered together as sisters to other Populus species.

SUBMITTER: Chen X

PROVIDER: S-EPMC7928004 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Sophora tonkinensis belongs to genus Sophora of the Fabaceae family. It is mainly distributed in the ridge and peak regions of limestone areas in western China and has high medicinal value and important ecological functions. Wild populations of S. tonkinensis are in danger and need urgent conservation. Furthermore, wild S. tonkinensis resources are very limited relative to the needs of the market, and many adulterants are present on the market. Therefore, a method for authenticating S. tonkinensis and its adulterants at the molecular level is needed. Chloroplast genomes are valuable sources of genetic markers for phylogenetic analyses, genetic diversity evaluation, and plant molecular identification. In this study, we report the complete chloroplast genome of S. tonkinensis. The circular complete chloroplast genome was 154,644 bp in length, containing an 85,810 bp long single-copy (LSC) region, an 18,321 bp short single-copy (SSC) region and two inverted repeat (IR) regions of 50,513 bp. The S. tonkinensis chloroplast genome comprised 129 genes, including 83 protein-coding genes, 38 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. The structure, gene order and guanine and cytosine (GC) content of the S. tonkinensis chloroplast genome were similar to those of the Sophora alopecuroides and Sophora flavescens chloroplast genomes. A total of 1,760 simple sequence repeats (SSRs) were identified in the chloroplast genome of S. tonkinensis, and most of them (93.1%) were mononucleotides. Moreover, the identified SSRs were mainly distributed in the LSC region, accounting for 60% of the total number of SSRs, while 316 (18%) and 383 (22%) were located in the SSC and IR regions, respectively. Only one complete copy of the rpl2 gene was present at the LSC/IRB boundary, while another copy was absent from the IRA region because of the incomplete structure caused by IR region expansion and contraction. The phylogenetic analysis placed S. tonkinensis in Papilionoideae, sister to S. flavescens, and the genera Sophora and Ammopiptanthus were closely related. The complete genome sequencing and chloroplast genome comparative analysis of S. tonkinensis and its closely related species presented in this paper will help formulate effective conservation and management strategies as well as molecular identification approaches for this important medicinal plant.

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The complete chloroplast genome sequence of Populus szechuanica, a medicinal plant for child anesthesia application.

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