Dataset Information

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea.

ABSTRACT: Potassium (K⁺) plays key roles in plant growth and development. However, molecular mechanism studies of K⁺ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K⁺ channel that is responsible for the long-distance transportation of K⁺ through xylem in roots. In this study, we determined a Shaker-type K⁺ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K⁺ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K⁺ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K⁺ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K⁺ transport and distribution in woody plants.

SUBMITTER: Chen Y

PROVIDER: S-EPMC7932800 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea.

Chen Yahui Y Peng Xuefeng X Cui Jijie J Zhang Hongxia H Jiang Jiang J Song Zhizhong Z

International journal of genomics 20210225

Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as Spu ...[more]

PMID: 33708988

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Project description:Background and aimsIncreasing energy demands and the necessity to reduce greenhouse gas emissions are key motivating factors driving the development of lignocellulosic crops as an alternative to non-renewable energy sources. The effects of global climate change will require a better understanding of the genetic basis of complex adaptive traits to breed more resilient bioenergy feedstocks, like willow (Salix spp.). Shrub willow is a sustainable and dedicated bioenergy crop, bred to be fast-growing and high-yielding on marginal land without competing with food crops. In a rapidly changing climate, genomic advances will be vital for the sustained improvement of willow and other non-model bioenergy crops. Here, joint genetic mapping was used to exploit genetic variation garnered from both recent and historical recombination events in S. purpurea.MethodsA panel of North American naturalized S. purpurea accessions and full-sib F2S. purpurea population were genotyped and phenotyped for a suite of morphological, physiological, pest and disease resistance, and wood chemical composition traits, collected from multi-environment and multi-year replicated field trials. Controlling for population stratification and kinship in the association panel and spatial variation in the F2, a comprehensive mixed model analysis was used to dissect the complex genetic architecture and plasticity of these important traits.Key resultsIndividually, genome-wide association (GWAS) models differed in terms of power, but the combined approach, which corrects for yearly and environmental co-factors across datasets, improved the overall detection and resolution of associated loci. Although there were few significant GWAS hits located within support intervals of QTL for corresponding traits in the F2, many large-effect QTL were identified, as well as QTL hotspots.ConclusionsThis study provides the first comparison of linkage analysis and linkage disequilibrium mapping approaches in Salix, and highlights the complementarity and limits of these two methods for elucidating the genetic architecture of complex bioenergy-related traits of a woody perennial breeding programme.

Dataset Information

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea.

Publications

Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, <i>Salix purpurea</i>.

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