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Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea.


ABSTRACT: Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K+ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K+ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K+ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K+ transport and distribution in woody plants.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7932800 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, <i>Salix purpurea</i>.

Chen Yahui Y   Peng Xuefeng X   Cui Jijie J   Zhang Hongxia H   Jiang Jiang J   Song Zhizhong Z  

International journal of genomics 20210225


Potassium (K<sup>+</sup>) plays key roles in plant growth and development. However, molecular mechanism studies of K<sup>+</sup> nutrition in forest plants are largely rare. In plants, <i>SKOR</i> gene encodes for the outward rectifying Shaker-type K<sup>+</sup> channel that is responsible for the long-distance transportation of K<sup>+</sup> through xylem in roots. In this study, we determined a Shaker-type K<sup>+</sup> channel gene in purple osier (<i>Salix purpurea</i>), designated as <i>Spu  ...[more]

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