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Aptamers and Aptamer-Coupled Biosensors to Detect Water-Borne Pathogens.

ABSTRACT: Aptamers can serve as efficient bioreceptors for the development of biosensing detection platforms. Aptamers are short DNA or RNA oligonucleotides that fold into specific structures, which enable them to selectively bind to target analytes. The method used to identify aptamers is Systematic Evolution of Ligands through Exponential Enrichment (SELEX). Target properties can have an impact on aptamer efficiencies. Therefore, characteristics of water-borne microbial targets must be carefully considered during SELEX for optimal aptamer development. Several aptamers have been described for key water-borne pathogens. Here, we provide an exhaustive overview of these aptamers and discuss important microbial aspects to consider when developing such aptamers.

SUBMITTER: Saad M

PROVIDER: S-EPMC7933031 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Aptamers and Aptamer-Coupled Biosensors to Detect Water-Borne Pathogens.

Saad Mariam M Faucher Sebastien P SP

Frontiers in microbiology 20210219

Aptamers can serve as efficient bioreceptors for the development of biosensing detection platforms. Aptamers are short DNA or RNA oligonucleotides that fold into specific structures, which enable them to selectively bind to target analytes. The method used to identify aptamers is Systematic Evolution of Ligands through Exponential Enrichment (SELEX). Target properties can have an impact on aptamer efficiencies. Therefore, characteristics of water-borne microbial targets must be carefully conside ...[more]

PMID: 33679681

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