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Prospects and challenges of using electrochemical immunosensors as an alternative detection method for SARS-CoV-2 wastewater-based epidemiology

ABSTRACT: Given its potential applications in confronting the COVID-19 pandemic, wastewater-based epidemiology (WBE) has attracted tremendous attention. Developing a fast, cost-effective, and practical method for SARS-CoV-2 detection in wastewater is of great significance to facilitate future WBE development. By now, the PCR-based approach serves as the reference method and “gold standard” to detect the virus in wastewater. However, we found a trend that the PCR-based method becomes almost an unshakable choice as more and more studies were published regarding SARS-CoV-2 WBE. Of note, the importance of exploring new, alternative approaches for SARS-CoV-2 detection in wastewater should not be underestimated. In this context, the prospect of using electrochemical immunosensors as the alternative detection method was investigated in this survey. Based on the previous efforts towards different virus immunoassay studies and newly published PCR-based COVD-19 WBE works, this survey provides new insights into the electrochemical immunoassay that have been widely adopted in body fluids virus detection, along with an extensive discussion of the detection mechanism, detection performance, past performances, current efforts, and potential challenges with wastewater detection. In the end, this survey concludes that using electrochemical immunosensors to analyze SARS-CoV-2 in wastewater samples quantitatively may have better feasibility and practicability than using the conventional PCR-based approach, especially when considering its fast detection, ease of miniaturization, and potential on-site measurement. Graphical abstract Unlabelled Image

SUBMITTER: Lu D

PROVIDER: S-EPMC7934662 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:With the economic and practical limits of medical screening for SARS-CoV-2/COVID-19 coming sharply into focus worldwide, scientists are turning now to wastewater-based epidemiology (WBE) as a potential tool for assessing and managing the pandemic. We employed computational analysis and modeling to examine the feasibility, economy, opportunities and challenges of enumerating active coronavirus infections locally and globally using WBE. Depending on local conditions, detection in community wastewater of one symptomatic/asymptomatic infected case per 100 to 2,000,000 non-infected people is theoretically feasible, with some practical successes now being reported from around the world. Computer simulations for past, present and emerging epidemic hotspots (e.g., Wuhan, Milan, Madrid, New York City, Teheran, Seattle, Detroit and New Orleans) identified temperature, average in-sewer travel time and per-capita water use as key variables. WBE surveillance of populations is shown to be orders of magnitude cheaper and faster than clinical screening, yet cannot fully replace it. Cost savings worldwide for one-time national surveillance campaigns are estimated to be in the million to billion US dollar range (US$), depending on a nation's population size and number of testing rounds conducted. For resource poor regions and nations, WBE may represent the only viable means of effective surveillance. Important limitations of WBE rest with its inability to identify individuals and to pinpoint their specific locations. Not compensating for temperature effects renders WBE data vulnerable to severe under-/over-estimation of infected cases. Effective surveillance may be envisioned as a two-step process in which WBE serves to identify and enumerate infected cases, where after clinical testing then serves to identify infected individuals in WBE-revealed hotspots. Data provided here demonstrate this approach to save money, be broadly applicable worldwide, and potentially aid in precision management of the pandemic, thereby helping to accelerate the global economic recovery that billions of people rely upon for their livelihoods.

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Prospects and challenges of using electrochemical immunosensors as an alternative detection method for SARS-CoV-2 wastewater-based epidemiology

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