Influence of Cell Quality on Inflammatory Biomarkers in COPD Sputum Supernatant.

ABSTRACT:

Purpose

We recently introduced a sputum cell quality score to rate how cell morphology, cellular debris and squamous cell contamination influence inflammatory cell identification during microscopic evaluation. However, sputum cell quality is generally not considered for the interpretation of sputum fluid phase biomarkers. Therefore, we compared the soluble protein concentrations between sputum samples with different cell quality. The impact of cell quality was compared to other factors potentially affecting soluble biomarker concentrations.

Methods

A comprehensive sputum dataset from 154 clinically stable COPD patients was used to analyse the differences and the variability of sputum supernatant concentrations for 23 proteins between low, medium, and high sputum cell quality

SUBMITTER: Pedersen F 

PROVIDER: S-EPMC7935341 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature