A simple quantitative PCR assay to determine TRAMP transgene zygosity.

ABSTRACT:

Background

The TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model remains one of the most widely used transgenic mouse models of prostate cancer. This is due to its ability to recapitulate with ~100% penetrance multiple aspects of the human disease such as prostatic intraepithelial neoplasia lesions, invasive carcinoma, progression to castration-resistant prostate cancer including aggressive neuroendocrine prostate cancer and metastasis. Despite its popularity, the use of TRAMP mice is limited/slowed by the inability to distinguish the zygosity of the TRAMP transgene. This is especially true for breeding strategies implementing multiple crosses and alleles and when the rapid generation of large animal cohorts with the desired genotype is needed.

Methods

We develop

SUBMITTER: Chen R 

PROVIDER: S-EPMC7936990 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature