Anti-diabetic effect of the lupinalbin A compound isolated from Apios americana: In vitro analysis and molecular docking study.

ABSTRACT: Dipeptidyl peptidase 4 (DPP4) and ?-glucosidase inhibitors have been developed as anti-diabetic agents for the treatment of diabetes mellitus. In the present study, the anti-diabetic effects of the lupinalbin A compound isolated from Apios americana was investigated by measuring its inhibitory activity against DPP4 and ?-glucosidase. To detect the inhibitory effect of lupinalbin A, DPP4 and ?-glucosidase assays were performed in vitro. Molecular docking analysis was performed using AutoDock 4.2. The IC₅₀ values of lupinalbin A against DPP4 and ?-glucosidase were 45.2 and 53.4 µM, respectively. Analysis of the enzyme kinetics revealed that lupinalbin A interacted with the active site of DPP4 in a competitive manner, with an inhibition constant (Ki) value of 35.1±2.0 µM, whereas the lupinalbin A interaction with ?-glucosidase was non-competitive, with a Ki value of 45.0 µM. Molecular docking analysis revealed a binding pose between the DPP4 enzyme and lupinalbin A. Taken together, these data suggest lupinalbin A is more effective against DPP4 than ?-glucosidase, with regard to its anti-diabetic effects.

SUBMITTER: Kim HY 

PROVIDER: S-EPMC7938295 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature