Dataset Information

IgG Epitopes Processed and Presented by IgG⁺ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

ABSTRACT: We described a human regulatory T cell (Treg) population activated by IgG⁺ B cells presenting peptides of the heavy C region (Fc) via processing of the surface IgG underlying a model for B cell-Treg cooperation in the human immune regulation. Functionally, Treg inhibited the polarization of naive T cells toward a proinflammatory phenotype in both a cognate and a noncognate fashion. Their fine specificities were similar in healthy donors and patients with rheumatoid arthritis, a systemic autoimmune disease. Four immunodominant Fc peptides bound multiple HLA class II alleles and were recognized by most subjects in the two cohorts. The presentation of Fc peptides that stimulate Treg through the processing of IgG by dendritic cells (DC) occurred in myeloid DC classical DC 1 and classical DC 2. Different routes of Ag processing of the IgG impacted Treg expansion in rheumatoid arthritis patients.

SUBMITTER: Hsieh LE

PROVIDER: S-EPMC7939040 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

IgG Epitopes Processed and Presented by IgG<sup>+</sup> B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

Hsieh Li-En LE Sidney John J Burns Jane C JC Boyle David L DL Firestein Gary S GS Altman Yoav Y Sette Alessandro A Franco Alessandra A

Journal of immunology (Baltimore, Md. : 1950) 20210212 6

We described a human regulatory T cell (Treg) population activated by IgG<sup>+</sup> B cells presenting peptides of the heavy C region (Fc) via processing of the surface IgG underlying a model for B cell-Treg cooperation in the human immune regulation. Functionally, Treg inhibited the polarization of naive T cells toward a proinflammatory phenotype in both a cognate and a noncognate fashion. Their fine specificities were similar in healthy donors and patients with rheumatoid arthritis, a system ...[more]

PMID: 33579724

Similar Datasets

Project description:Apremilast is a novel phosphodiesterase 4 (PDE4) inhibitor suppressing immune and inflammatory responses. We assessed the anti-inflammatory effects of Apremilast in type II collagen (CII)-induced arthritis (CIA) mouse model. To determine whether Apremilast can ameliorate arthritis onset in this model, Apremilast was given orally at day 14 after CII immunization. Bone erosion was measured by histological and micro-computed tomographic analysis. Anti-mouse CII antibody levels were measured by enzyme-linked immunosorbent assay, and Th17, Th1 cells, and CD4+Foxp3+ regulatory T (Treg) cells were assessed by flow cytometry in the lymph nodes. Human cartilage and rheumatoid arthritis (RA) synovial fibroblasts (RASFs) implantation in the severe combined immunodeficiency mouse model of RA were used to study the role of Apremilast in the suppression of RASF-mediated cartilage destruction in vivo. Compared with untreated and vehicle control groups, we found that Apremilast therapy delayed arthritis onset and reduced arthritis scores in the CIA model. Total serum IgG, IgG1, IgG2a, and IgG2b were all decreased in the Apremilast treatment groups. Moreover, Apremilast markedly prevented the development of bone erosions in CIA mice by CT analysis. Furthermore, in the Apremilast treated group, the frequency of Th17 cells and Th1 cells was significantly decreased while Treg cells' frequency was significantly increased. The high dose of Apremilast (25 mg/kg) was superior to low dose (5 mg/kg) in treating CIA. Apremilast treatment reduced the migratory ability of RASFs and their destructive effect on cartilage. Compared with the model group, Apremilast treatment significantly reduced the RASFs invasion cartilage scores in both primary implant and contralateral implant models. Our data suggest that Apremilast is effective in treating autoimmune arthritis and preventing the bone erosion in the CIA model, implicating its therapeutic potential in patients with RA.

Dataset Information

IgG Epitopes Processed and Presented by IgG⁺ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

Publications

IgG Epitopes Processed and Presented by IgG<sup>+</sup> B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

IgG Epitopes Processed and Presented by IgG+ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

Publications

IgG Epitopes Processed and Presented by IgG<sup>+</sup> B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

IgG Epitopes Processed and Presented by IgG⁺ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells.