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Enhanced collection of phenotypic and engrafting human cord blood hematopoietic stem cells at 4°C.

ABSTRACT: The number of hematopoietic stem cells (HSCs) collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self-renewing NSG immune-deficient mouse engrafting and SCID-repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as noted previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost-effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.

SUBMITTER: Broxmeyer HE

PROVIDER: S-EPMC7941408 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Enhanced collection of phenotypic and engrafting human cord blood hematopoietic stem cells at 4°C.

Broxmeyer Hal E HE Cooper Scott S Capitano Maegan L ML

Stem cells (Dayton, Ohio) 20200704 10

The number of hematopoietic stem cells (HSCs) collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self-renewing NSG immune-deficient mouse engrafting and SCID-repopulating cells. This was associated with decreased numbers ...[more]

PMID: 32535978

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