Dataset Information

Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo.

ABSTRACT:

Background

PGF2? is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2?. EGR1 is involved in the transactivation of many genes, including TGF?1, which plays an important role during luteal regression.

Methods

The current study was conducted in buffalo luteal cells with the aim to better understand the role of EGR1 in transactivation of TGF?1 during PGF2? induced luteal regression. Luteal cells from mid stage corpus luteum of buffalo were cultured and treated with different doses of PGF2? for different time durations. Relative expression of mRNAs encoding for enzymes within the progesterone biosynthetic pathway (3?HSD, CYP11A1 and StAR); Caspase 3; AKT were analyzed to confirm the occurrence of luteolytic event. To determine if EGR1 is involved in the PGF2? induced luteal regression via induction of TGF?1 expression, we knocked out the EGR1 gene by using CRISPR/Cas9.

Result

The present experiment determined whether EGR1 protein expression in luteal cells was responsive to PGF2? treatment. Quantification of EGR1 and TGF?1 mRNA showed significant up regulation in luteal cells of buffalo at 12 h post PGF2? induction. In order to validate the role of PGF2? on stimulating the expression of TGF?1 by an EGR1 dependent mechanism we knocked out EGR1. The EGR1 ablated luteal cells were stimulated with PGF2? and it was observed that EGR1 KO did not modulate the PGF2? induced expression of TGF?1. In PGF2? treated EGR1 KO luteal cell, the mRNA expression of Caspase 3 was significantly increased compared to PGF2? treated wild type luteal cells maintained for 12 h. We also studied the influence of EGR1 on steroidogenesis. The EGR1 KO luteal cells with PGF2? treatment showed no substantial difference either in the progesterone concentration or in StAR mRNA expression with PGF2?-treated wild type luteal cells.

Conclusion

These results suggest that EGR1 signaling is not the only factor which plays a role in the regulation of PGF2? induced TGF?1 signaling for luteolysis.

SUBMITTER: Punetha M

PROVIDER: S-EPMC7953609 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Publications

Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo.

Punetha Meeti M Kumar Sai S Paul Avishek A Jose Bosco B Bharati Jaya J Sonwane Arvind A Green Jonathan A JA Whitworth Kristin K Sarkar Mihir M

Biological research 20210312 1

<h4>Background</h4>PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2α. EGR1 is involved in the transactivation of many genes, including TGFβ1, which plays an important role during luteal regression.<h4> ...[more]

PMID: 33712084

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Dataset Information

Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo.

Background

Methods

Result

Conclusion

Publications

Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo.

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