Dataset Information

Fitness characteristics of the malaria vector Anopheles funestus during an attempted laboratory colonization.

ABSTRACT:

Background

The malaria vector Anopheles funestus is increasingly recognized as a dominant vector of residual transmission in many African settings. Efforts to better understand its biology and control are significantly impeded by the difficulties of colonizing it under laboratory conditions. To identify key bottlenecks in colonization, this study compared the development and fitness characteristics of wild An. funestus from Tanzania (FUTAZ) and their F₁ offspring during colonization attempts. The demography and reproductive success of wild FUTAZ offspring were compared to that of individuals from one of the only An. funestus strains that has been successfully colonized (FUMOZ, from Mozambique) under similar laboratory conditions.

Methods

Wild An. funestus (FUTAZ) were collected from three Tanzanian villages and maintained inside an insectary at 70-85% RH, 25-27 °C and 12 h:12 h photoperiod. Eggs from these females were used to establish three replicate F₁ laboratory generations. Larval development, survival, fecundity, mating success, percentage pupation and wing length were measured in the F₁ -FUTAZ offspring and compared with wild FUTAZ and FUMOZ mosquitoes.

Results

Wild FUTAZ laid fewer eggs (64.1; 95% CI [63.2, 65.0]) than FUMOZ females (76.1; 95% CI [73.3, 79.1]). Survival of F₁-FUTAZ larvae under laboratory conditions was low, with an egg-to-pupae conversion rate of only 5.9% compared to 27.4% in FUMOZ. The median lifespan of F₁-FUTAZ females (32 days) and males (33 days) was lower than FUMOZ (52 and 49 for females and males respectively). The proportion of female F₁-FUTAZ inseminated under laboratory conditions (9%) was considerably lower than either FUMOZ (72%) or wild-caught FUTAZ females (92%). This resulted in nearly zero viable F₂-FUTAZ eggs produced. Wild FUTAZ wings appear to be larger compared to the lab reared F₁-FUTAZ and FUMOZ.

Conclusions

This study indicates that poor larval survival, mating success, low fecundity and shorter survival under laboratory conditions all contribute to difficulties in colonizing of An. funestus. Future studies should focus on enhancing these aspects of An. funestus fitness in the laboratory, with the biggest barrier likely to be poor mating.

SUBMITTER: Ngowo HS

PROVIDER: S-EPMC7955623 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fitness characteristics of the malaria vector Anopheles funestus during an attempted laboratory colonization.

Ngowo Halfan S HS Hape Emmanuel E EE Matthiopoulos Jason J Ferguson Heather M HM Okumu Fredros O FO

Malaria journal 20210312 1

<h4>Background</h4>The malaria vector Anopheles funestus is increasingly recognized as a dominant vector of residual transmission in many African settings. Efforts to better understand its biology and control are significantly impeded by the difficulties of colonizing it under laboratory conditions. To identify key bottlenecks in colonization, this study compared the development and fitness characteristics of wild An. funestus from Tanzania (FUTAZ) and their F<sub>1</sub> offspring during coloni ...[more]

PMID: 33712003

Similar Datasets

Project description:BackgroundMalaria affects large parts of the developing world and is responsible for almost 800,000 deaths annually. As climates change, concerns have arisen as to how this vector-borne disease will be impacted by changing rainfall patterns and warming temperatures. Despite the importance and controversy surrounding the impact of climate change on the potential spread of this disease, little information exists on the tolerances of several of the vector species themselves.MethodsUsing a ramping protocol (to assess critical thermal limits--CT) and plunge protocol (to assess lethal temperature limits--LT) information on the thermal tolerance of two of Africa's important malaria vectors, Anopheles arabiensis and Anopheles funestus was collected. The effects of age, thermal acclimation treatment, sex and strain (laboratory versus wild adults) were investigated for CT determinations for each species. The effects of age and sex for adults and life stage (larvae, pupae, adults) were investigated for LT determinations.ResultsIn both species, females are more tolerant to low and high temperatures than males; larvae and pupae have higher upper lethal limits than do adults. Thermal acclimation of adults has large effects in some instances but small effects in others. Younger adults tend to be more tolerant of low or high temperatures than older age groups. Long-standing laboratory colonies are sufficiently similar in thermal tolerance to field-collected animals to provide reasonable surrogates when making inferences about wild population responses. Differences between these two vectors in their thermal tolerances, especially in larvae and pupae, are plausibly a consequence of different habitat utilization.ConclusionsLimited plasticity is characteristic of the adults of these vector species relative to others examined to date, suggesting limited scope for within-generation change in thermal tolerance. These findings and the greater tolerance of females to thermal extremes may have significant implications for future malaria transmission, especially in areas of current seasonal transmission and in areas on the boundaries of current vector distribution.

Dataset Information

Fitness characteristics of the malaria vector Anopheles funestus during an attempted laboratory colonization.

Background

Methods

Results

Conclusions

Publications

Fitness characteristics of the malaria vector Anopheles funestus during an attempted laboratory colonization.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets