Dataset Information

Influence of Cu²⁺ on Osteoclast Formation and Activity In Vitro.

ABSTRACT:

Background

Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu²⁺ on osteoclasts, which play a major role in bone remodeling was studied in detail.

Methods

Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu²⁺. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu²⁺.

Results

Up to 8 µM Cu²⁺ did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu²⁺ but decreased in the presence of extracellular bone matrix. When Cu²⁺ was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu²⁺.

Conclusions

Differentiating and mature osteoclasts react differently to Cu²⁺. High TRAP activities are not necessarily related to high resorption.

SUBMITTER: Bernhardt A

PROVIDER: S-EPMC7957576 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Influence of Cu<sup>2+</sup> on Osteoclast Formation and Activity In Vitro.

Bernhardt Anne A Bacova Jana J Gbureck Uwe U Gelinsky Michael M

International journal of molecular sciences 20210228 5

<h4>Background</h4>Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu<sup>2+</sup> on osteoclasts, which play a major role in bone remodeling was studied in detail.<h4>Methods</h4>Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu<sup>2+</sup>. Osteoclast formation and activity were analyzed by mea ...[more]

PMID: 33671069

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Dataset Information

Influence of Cu²⁺ on Osteoclast Formation and Activity In Vitro.

Background

Methods

Results

Conclusions

Publications

Influence of Cu<sup>2+</sup> on Osteoclast Formation and Activity In Vitro.

Similar Datasets

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Dataset Information

Influence of Cu2+ on Osteoclast Formation and Activity In Vitro.

Background

Methods

Results

Conclusions

Publications

Influence of Cu<sup>2+</sup> on Osteoclast Formation and Activity In Vitro.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Influence of Cu²⁺ on Osteoclast Formation and Activity In Vitro.