Unknown

Dataset Information

0

Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests.


ABSTRACT: We report an electrochemical biosensor combined with recombinase polymerase amplification (RPA) for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2. The electrochemical biosensor based on a multi-microelectrode array allows the detection of multiple target genes by differential pulse voltammetry. The RPA reaction involves hybridization of the RPA amplicon with thiol-modified primers immobilized on the working electrodes, which leads to a reduction of current density as amplicons accumulate. The assay results in shorter "sample-to-answer" times than conventional PCR without expensive thermo-cycling equipment. The limits of detection are about 0.972 fg/μL (RdRP gene) and 3.925 fg/μL (N gene), which are slightly lower than or comparable to that of RPA assay results obtained by gel electrophoresis without post-amplification purification. The combination of electrochemical biosensors and the RPA assay is a rapid, sensitive, and convenient platform that can be potentially used as a point-of-care test for the diagnosis of COVID-19.

SUBMITTER: Kim HE 

PROVIDER: S-EPMC7970423 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3509743 | biostudies-literature
| S-EPMC10080115 | biostudies-literature
| S-EPMC6679679 | biostudies-literature
| S-EPMC7361114 | biostudies-literature
| S-EPMC9270181 | biostudies-literature
| S-EPMC7464797 | biostudies-literature
| S-EPMC8636779 | biostudies-literature
| S-EPMC5240331 | biostudies-literature
| S-EPMC9132313 | biostudies-literature
| S-EPMC9318599 | biostudies-literature