Dataset Information

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential.

ABSTRACT:

Background

Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach.

Methods

Streptomyces strains' growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC₅₀ values were calculated. Metabolomics analysis was conducted on both strains' mid-exponential and stationary phase extracts.

Results

The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC₅₀ values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC₅₀ value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done.

SUBMITTER: Ahmad SJ

PROVIDER: S-EPMC7971094 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Metabolite profiling of endophytic <i>Streptomyces</i> spp. and its antiplasmodial potential.

Ahmad Siti Junaidah SJ Mohamad Zin Noraziah N Mazlan Noor Wini NW Baharum Syarul Nataqain SN Baba Mohd Shukri MS Lau Yee Ling YL

PeerJ 20210315

<h4>Background</h4>Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of <i>Streptomyces</i> spp. against <i>Plasmodium falciparum</i> 3D7 by using a metabolomics approach.<h4>Methods</h4><i>Streptomyces</i> strains' growth curves, namely SUK 12 and SUK 48, were measured and <i>P</i>. <i>falciparum</i> 3D7 IC<sub>50</sub> values were calculated. Metabolomics analysis was conducted on bot ...[more]

PMID: 33777509

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