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Stokesian dynamics simulations of a magnetotactic bacterium.

ABSTRACT: The swimming of bacteria provides insight into propulsion and steering under the conditions of low-Reynolds number hydrodynamics. Here we address the magnetically steered swimming of magnetotactic bacteria. We use Stokesian dynamics simulations to study the swimming of single-flagellated magnetotactic bacteria (MTB) in an external magnetic field. Our model MTB consists of a spherical cell body equipped with a magnetic dipole moment and a helical flagellum rotated by a rotary motor. The elasticity of the flagellum as well as magnetic and hydrodynamic interactions is taken into account in this model. We characterized how the swimming velocity is dependent on parameters of the model. We then studied the U-turn motion after a field reversal and found two regimes for weak and strong fields and, correspondingly, two characteristic time scales. In the two regimes, the U-turn time is dominated by the turning of the cell body and its magnetic moment or the turning of the flagellum, respectively. In the regime for weak fields, where turning is dominated by the magnetic relaxation, the U-turn time is approximately in agreement with a theoretical model based on torque balance. In the strong-field regime, strong deformations of the flagellum are observed. We further simulated the swimming of a bacterium with a magnetic moment that is inclined relative to the flagellar axis. This scenario leads to intriguing double helical trajectories that we characterize as functions of the magnetic moment inclination and the magnetic field. For small inclination angles ([Formula: see text]) and typical field strengths, the inclination of the magnetic moment has only a minor effect on the swimming of MTB in an external magnetic field. Large inclination angles result in a strong reduction in the velocity in direction of the magnetic field, consistent with recent observations that bacteria with large inclination angles use a different propulsion mechanism.

SUBMITTER: Mohammadinejad S

PROVIDER: S-EPMC7987682 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:UnlabelledMagnetotactic bacteria biosynthesize specific organelles, the magnetosomes, which are membrane-enclosed crystals of a magnetic iron mineral that are aligned in a linear chain. The number and size of magnetosome particles have to be critically controlled to build a sensor sufficiently strong to ensure the efficient alignment of cells within Earth's weak magnetic field while at the same time minimizing the metabolic costs imposed by excessive magnetosome biosynthesis. Apart from their biological function, bacterial magnetosomes have gained considerable interest since they provide a highly useful model for prokaryotic organelle formation and represent biogenic magnetic nanoparticles with exceptional properties. However, potential applications have been hampered by the difficult cultivation of these fastidious bacteria and their poor yields of magnetosomes. In this study, we found that the size and number of magnetosomes within the cell are controlled by many different Mam and Mms proteins. We present a strategy for the overexpression of magnetosome biosynthesis genes in the alphaproteobacterium Magnetospirillum gryphiswaldense by chromosomal multiplication of individual and multiple magnetosome gene clusters via transposition. While stepwise amplification of the mms6 operon resulted in the formation of increasingly larger crystals (increase of ?35%), the duplication of all major magnetosome operons (mamGFDC, mamAB, mms6, and mamXY, comprising 29 genes in total) yielded an overproducing strain in which magnetosome numbers were 2.2-fold increased. We demonstrate that the tuned expression of the mam and mms clusters provides a powerful strategy for the control of magnetosome size and number, thereby setting the stage for high-yield production of tailored magnetic nanoparticles by synthetic biology approaches.ImportanceBefore our study, it had remained unknown how the upper sizes and numbers of magnetosomes are genetically regulated, and overproduction of magnetosome biosynthesis had not been achieved, owing to the difficulties of large-scale genome engineering in the recalcitrant magnetotactic bacteria. In this study, we established and systematically explored a strategy for the overexpression of magnetosome biosynthesis genes by genomic amplification of single and multiple magnetosome gene clusters via sequential chromosomal insertion by transposition. Our findings also indicate that the expression levels of magnetosome proteins together limit the upper size and number of magnetosomes within the cell. We demonstrate that tuned overexpression of magnetosome gene clusters provides a powerful strategy for the precise control of magnetosome size and number.

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Stokesian dynamics simulations of a magnetotactic bacterium.

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