Let-7 mediated airway remodelling in chronic obstructive pulmonary disease via the regulation of IL-6.

ABSTRACT:

Background

Myofibroblast differentiation and extracellular matrix (ECM) deposition are observed in chronic obstructive pulmonary disease (COPD). However, the mechanisms of regulation of myofibroblast differentiation remain unclear.

Materials and methods

We detected let-7 levels in peripheral lung tissues, serum and primary bronchial epithelial cells of COPD patients and cigarette smoke (CS)-exposed mice. IL-6 mRNA was explored in lung tissues of COPD patients and CS-exposed mice. IL-6 protein was detected in cell supernatant from primary epithelial cells by ELISA. We confirmed the regulatory effect of let-7 on IL-6 by luciferase reporter assay. Western blotting assay was used to determine the expression of α-SMA, E-cadherin and collagen I. In vitro, cell study was performed

SUBMITTER: Di T 

PROVIDER: S-EPMC7988621 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature