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Pesticide interactions and risks of sperm chromosomal abnormalities.

ABSTRACT: Disentangling the separate and synergistic effects of chemicals poses methodological challenges for accurate exposure assessment and for investigating epidemiologically how chemicals affect reproduction. We investigated combined exposures to ubiquitous contemporary use pesticides, specifically organophosphates (OP) and pyrethroids (PYR), and their association with germ cell abnormalities among adult men. Fluorescence in situ hybridization was used to determine disomy in sperm nuclei and urine was analyzed for concentrations of PYR metabolites (3-phenoxybenzoic acid; 3PBA) and OP dialkyl phosphate (DAP) metabolites. Incidence rate ratios using Poisson models were estimated for each disomy type by exposure quartile of DAP metabolites and 3PBA, controlling for confounders. The shape of the associations between PYRs, OPs and disomy were frequently nonmonotonic. There were consistent interactions between OP and PYR metabolite concentrations and the risk for sperm abnormalities. Taking both chemicals into account simultaneously resulted in quantitatively different associations than what was reported previously for OPs and PYRs separately, demonstrating the importance of modeling multiple concentrations simultaneously. Methods investigating interactions using Poisson models are needed to better quantify chemical interactions and their effects on count-based health outcomes, the importance of which was shown here for germ cell abnormalities.

SUBMITTER: Figueroa ZI

PROVIDER: S-EPMC7994026 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Transmitted de novo structural chromosomal abnormalities, the majority of which are paternally derived, can lead to abnormal reproductive outcomes as well as genetic diseases in offspring. We developed and validated a new multicolor FISH procedure (sperm ACM, which utilizes DNA probes specific for the alpha [1cen], classical, [1q12], and midi [1p36.3] satellites of chromosome 1) which utilizes DNA probes specific for three regions of chromosome 1 to detect human sperm that carry numerical abnormalities plus two categories of structural aberrations: (1) duplications and deletions of 1pter and 1cen, and (2) chromosomal breaks within the 1cen-1q12 region. In healthy men, the average frequencies of sperm with duplications and deletions were (a) 4.5 +/- 0.5 and 4.1 +/- 1.3 per 10(4) involving 1pter and (b) 0.9 +/- 0.4 and 0.8 +/- 0.3 per 10(4) involving 1cen, respectively. The frequency of sperm exhibiting breaks within the 1cen-1q12 region was 14.1 +/- 1.2 per 10(4). Structural aberrations accounted for 71% of the abnormalities detected by sperm ACM, which was significantly higher than numerical abnormalities (P=2x10-8). Our findings also suggest that, for healthy men, (a) sperm carrying postmeiotic chromosomal breaks appear to be more prevalent than those carrying products of premeiotic or meiotic breakage or rearrangements, (b) the high frequency of chromosome breaks measured after "fertilization" by the hamster-egg cytogenetic method already appear to be present and detectable within human sperm by FISH, and (c) there are nonrandom and donor-specific distributions of breakpoint locations within 1q12 in sperm. FISH facilitates the analysis of much larger numbers of sperm than was possible when the hamster-egg method was used. Therefore, FISH-based procedures for simultaneously detecting chromosomal breaks, rearrangements, and numerical abnormalities in sperm may have widespread applications in human genetics, genetic toxicology, and reproductive medicine.

Project description:The biological importance of calcifications occasionally noted in fetal tissues (mainly liver) at autopsy or ultrasound is largely unexplored. Previous reports hint at an association to infection, circulatory compromise, malformations or chromosomal abnormalities. To identify factors associated with calcifications, we have performed a case-control study on the largest cohort of fetuses with calcifications described thus far.One-hundred and fifty-one fetuses with calcifications and 302 matched controls were selected from the archives of the Department of Pathology, Karolinska University Hospital. Chromosome analysis by karyotyping or quantitative fluorescence-polymerase chain reaction was performed. Autopsy and placenta reports were scrutinized for presence of malformations and signs of infection.Calcifications were mainly located in the liver, but also in heart, bowel, and other tissues. Fetuses with calcifications showed a significantly higher proportion of chromosomal abnormalities than controls; 50% vs. 20% (p<0.001). The most frequent aberrations among cases included trisomy 21 (33%), trisomy 18 (22%), and monosomy X (18%). A similar distribution was seen among controls. When comparing cases and controls with chromosomal abnormalities, the cases had a significantly higher prevalence of malformations (95% vs. 77%, p=0.004). Analyzed the other way around, cases with malformations had a significantly higher proportion of chromosomal abnormalities compared with controls, (66% vs. 31%, p<0.001).The presence of fetal calcifications is associated with high risk of chromosomal abnormality in combination with malformations. Identification of a calcification together with a malformation at autopsy more than doubles the probability of detecting a chromosomal abnormality, compared with identification of a malformation only. We propose that identification of a fetal tissue calcification at autopsy, and potentially also at ultrasound examination, should infer special attention towards co-existence of malformations, as this would be a strong indicator for a chromosomal abnormality.

Project description:Background.Population growth has led to the need to increase global food production. Pesticides are an important tool used in efforts to control insect pests. About 20–30% of agricultural produce is lost annually due to insect pests, diseases, weeds and rodents. While pesticides are effective against pest populations, if used injudiciously, they may pose health hazards to humans, domestic animals, natural enemies of crop pests and other forms of life through unwanted contamination of food, feed, water bodies and the environment.Objectives.The aim of the present study was to examine farmers' level of knowledge and awareness of environmental pollution due to unsafe use of pesticides for controlling rice pests and to explore ways of reducing their usage.Methods.The research population consisted of 120 rice farmers: 40 farmers randomly selected from each of the three rice growing districts of Bangladesh; Mymensingh, Tangail and Jamalpur. Data was collected through group discussions, direct observations and personal interviews during March 2013 to May 2014.Results.Farmers mainly sought advice on pesticide use from pesticide dealers or retailers and a very few farmers contacted government extension workers for this purpose. Most of the farmers had an understanding of natural enemies of rice pests and that the application of synthetic insecticides in the field can reduce their population. A few farmers followed integrated pest management (IPM) practices with little understanding of the adverse effects of insecticides on the environment and ecosystem. The majority of farmers understood the harmful effects of pesticides on health of human and animals, beneficial species, fish, insect resistance, soil and food. It was also found that education on pest management, information through television, more contact with extension personnel and farmers' awareness of IPM were critical factors for improving rice farmers' understanding of the ecological hazards caused by overuse of pesticides. Most of the farmers suggested that timely removal of weeds, appropriate timing for pesticide application, balanced doses of fertilizers, pest monitoring, correct dose of appropriate pesticides, pest tolerant varieties, increasing technical knowledge and skills and creating social awareness of environmental pollution among farmers were necessary to reduce the quantity of pesticides and minimize environmental hazards.Conclusions.The present study identified a need to intensify farmers' awareness and knowledge of integrated pest management and environmental pollution through extension organizations which could help promote sustainable agricultural development and improve the currently endangered bio-diversity of Bangladesh.Participant Consent.ObtainedEthical Approval.This study was approved by the PhD Supervisory Committee assigned by the Department of Entomology of Bangladesh Agricultural University.Competing Interests.The authors declare no competing financial interests

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Pesticide interactions and risks of sperm chromosomal abnormalities.

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