Dolichos kongkandae sp. nov. and lectotypification of D. fragrans (Leguminosae, Papilionoideae) from Asia.
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ABSTRACT:
SUBMITTER: Meeboonya R
PROVIDER: S-EPMC7997857 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Background and aimsSpecies of the genus Burkholderia, from the Betaproteobacteria, have been isolated from legume nodules, but so far they have only been shown to form symbioses with species of Mimosa, sub-family Mimosoideae. This work investigates whether Burkholderia tuberum strains STM678 (isolated from Aspalathus carnosa) and DUS833 (from Aspalathus callosa) can nodulate species of the South African endemic papilionoid genera Cyclopia (tribe Podalyrieae) and Aspalathus (Crotalarieae) as well as the promiscuous legume Macroptilium atropurpureum (Phaseoleae).MethodsBacterial strains and the phylogeny of their symbiosis-related (nod) genes were examined via 16S rRNA gene sequencing. Seedlings were grown in liquid culture and inoculated with one of the two strains of B. tuberum or with Sinorhizobium strain NGR 234 (from Lablab purpureus), Mesorhizobium strain DUS835 (from Aspalathus linearis) or Methylobacterium nodulans (from Crotalaria podocarpa). Some nodules, inoculated with green fluorescence protein (GFP)-tagged strains, were examined by light and electron microscopy coupled with immunogold labelling with a Burkholderia-specific antibody. The presence of active nitrogenase was checked by immunolabelling of nitrogenase and by the acetylene reduction assay. B. tuberum STM678 was also tested on a wide range of legumes from all three sub-families.Key resultsNodules were not formed on any of the Aspalathus spp. Only B. tuberum nodulated Cyclopia falcata, C. galioides, C. genistoides, C. intermedia and C. pubescens. It also effectively nodulated M. atropurpureum but no other species tested. GFP-expressing inoculant strains were located inside infected cells of C. genistoides, and bacteroids in both Cyclopia spp. and M. atropurpureum were immunogold-labelled with antibodies against Burkholderia and nitrogenase. Nitrogenase activity was also shown using the acetylene reduction assay. This is the first demonstration that a beta-rhizobial strain can effectively nodulate papilioinoid legumes.ConclusionsPapilionoid legumes from widely different tribes can be nodulated by beta-rhizobia, forming both indeterminate (Cyclopia) and determinate (Macroptilium) nodules.
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Project description:Stylosanthes (Papilionoideae, Leguminosae) is a predominantly Neotropical genus with ~48 species that include worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephala, and S. ruellioides). In addition, staining with CMA and DAPI, in situ hybridization with 5S and 35S rDNA probes, and estimation of DNA content were performed. The interpretation of Stylosanthes chromosome diversification was anchored by a comparison with the sister genus Arachis and a dated molecular phylogeny based on nuclear and plastid loci. Stylosanthes species showed 2n = 20, with low cytomolecular diversification regarding 5S rDNA, 35S rDNA, and genome size. Arachis has a more ancient diversification (~7 Mya in the Pliocene) than the relatively recent Stylosanthes (~2 Mya in the Pleistocene), and it seems more diverse than its sister lineage. Our data support the idea that the cytomolecular stability of Stylosanthes in relation to Arachis could be a result of its recent origin. The recent diversification of Stylosanthes could also be related to the low morphological differentiation among species, and to the recurrent formation of allopolyploid complexes.
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Project description:Morphological characters currently used to differentiate Caraganaopulens as a species have been found to be insufficient and inconsistent. Through extensive research and comparisons of specimens, it has been revealed that C.opulens and its synonyms have overlapping geographical distributions, and that typification is necessary for C.opulens. Therefore, a lectotype is designated for the name C.opulens, with comments on its typification. Additionally, the current typification status is discussed for all its synonyms, accompanied by substantive notes.
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Project description:The Millettioid/Phaseoloid (MP) clade from the subfamily Papilionoideae (Leguminosae) consists of six tribes and ca. 3,000 species. Previous studies have revealed some plastome structural variations (PSVs) within this clade. However, many deep evolutionary relationships within the clade remain unresolved. Due to limited taxon sampling and few genetic markers in previous studies, our understanding of the evolutionary history of this clade is limited. To address this issue, we sampled 43 plastomes (35 newly sequenced) representing all the six tribes of the MP clade to examine genomic structural variations and phylogenetic relationships. Plastomes of the species from the MP clade were typically quadripartite (size ranged from 140,029 to 160,040 bp) and contained 109-111 unique genes. We revealed four independent gene losses (ndhF, psbI, rps16, and trnS-GCU), multiple IR-SC boundary shifts, and six inversions in the tribes Desmodieae, Millettieae, and Phaseoleae. Plastomes of the species from the MP clade have experienced significant variations which provide valuable information on the evolution of the clade. Plastid phylogenomic analyses using Maximum Likelihood and Bayesian methods yielded a well-resolved phylogeny at the tribal and generic levels within the MP clade. This result indicates that plastome data is useful and reliable data for resolving the evolutionary relationships of the MP clade. This study provides new insights into the phylogenetic relationships and PSVs within this clade.
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Project description:The Dioclea clade comprises four genera and aproximately 60 species of the tribe Diocleae: Cleobulia (4 species), Cymbosema (1), Dioclea (ca. 50), Luzonia (1) and Macropsychanthus (3-4). Dioclea has been demonstrated to be a non-monophyletic genus, but low sampling in previous phylogenetic studies hampered the adoption of new taxonomic arrangements. We carried out densely sampled phylogenetic analyses of the Dioclea clade using molecular markers that had performed well in previous studies: the ITS and ETS nuclear ribosomal regions and the plastid trnK/matK. Our results support the maintenance of the genera Cleobulia and Cymbosema with their current circumscriptions, but confirmed the polyphyly of Dioclea, with its species falling into three different positions: (1) the puzzling species, Dioclea paniculata, was highly supported as a member of the Galactia clade; (2) Dioclea subg. Dioclea appeared as sister to a clade composed of Cleobulia and Cymbosema; and (3) the species of Dioclea subgenera Pachylobium and Platylobium composed a paraphyletic grade nesting the genera Luzonia and Macropsychanthus. We thus propose that the circumscription of Dioclea should be restricted to Dioclea subg. Dioclea, with 13 species and that the limits of Macropsychanthus should be widened to include the genus Luzonia, as well as the Dioclea subgenera Pachylobium and Platylobium, with 46 species. Taxonomic summaries, new combinations and synonyms are presented for all genera of the Dioclea clade. Cleobulia and Cymbosema were retained in their original circumscriptions. We presented an illustrated taxonomic conspectus of all genera of the Dioclea clade including 44 new combinations, one new name, ten new synonyms, two re-established holotypes, 38 lectotypes, two epitypes and one neotype.
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