Unknown

Dataset Information

0

Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model.


ABSTRACT: The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs' chemical functionalization and suitable for long-term culture of human Bone Marrow-Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

SUBMITTER: Argentati C 

PROVIDER: S-EPMC8003255 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

2017-09-23 | GSE98236 | GEO
| S-EPMC6350623 | biostudies-literature
| S-EPMC5772366 | biostudies-literature
| PRJEB23155 | ENA
| S-EPMC4451868 | biostudies-other
| S-EPMC5701218 | biostudies-literature
| S-EPMC4782980 | biostudies-literature
| S-EPMC6643057 | biostudies-literature
| S-EPMC7956555 | biostudies-literature
| S-EPMC9457856 | biostudies-literature