Direct PCR amplification from saliva sample using non-direct multiplex STR kits for forensic DNA typing.

ABSTRACT: Due to its proficiency to provide the most discriminating results for forensic applications, medical research and anthropological studies, multiplex PCR based STR analysis has been established as the most efficient technique in the forensic DNA analysis. Several multiplex amplification kits based on 4, 5 and 6 dyes chemistry are commercially available and used in forensic DNA typing across the globe. These multiplex PCR systems are routinely used for amplification of multiple STR loci (Autosomal, Y and/or X STR's) in the DNA extracted from various biological samples. In the routine forensic DNA testing, DNA profile obtained is compared with the DNA profile of the reference sample, which takes a certain turnaround time and employs costly lab resources. Successive development in forensic DNA

SUBMITTER: Shrivastava P 

PROVIDER: S-EPMC8007628 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature