Dataset Information

Complete mitochondrial genome and the phylogenetic position of the Burmese narrow-headed softshell turtle Chitra vandijki (Testudines: Trionychidae).

ABSTRACT: Narrow-headed softshell turtles constitute a group of critically endangered freshwater turtles that belong to the family Trionychidae. Here, we determine the complete mitogenome of the Burmese narrow-headed softshell turtle Chitra vandijki. The length of the mitochondrial genome was 16,614 bp, composed of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and twelve noncoding regions. The phylogenetic analysis strongly indicated that C. vandijki is closely related to C. indica. The mitochondrial genome will contribute to the genetic research and conservation of C. vandijki in the future.

SUBMITTER: Chen C

PROVIDER: S-EPMC8008941 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The characterization of mitochondrial genome has been evidenced as an efficient field of study for phylogenetic and evolutionary analysis in vertebrates including turtles. The aim of this study was to distinguish the structure and variability of the Trionychidae species mitogenomes through comparative analysis. The complete mitogenome (16796 bp) of an endangered freshwater turtle, Nilssonia nigricans was sequenced and annotated. The mitogenome encoded for 37 genes and a major non-coding control region (CR). The mitogenome was A + T biased (62.16%) and included six overlapping and 19 intergenic spacer regions. The Relative synonymous codon usage (RSCU) value was consistent among all the Trionychidae species; with the exception of significant reduction of Serine (TCG) frequency in N. nigricans, N. formosa, and R. swinhoei. In N. nigricans, most of the transfer RNAs (tRNAs) were folded into classic clover-leaf secondary structures with Watson-Crick base pairing except for trnS1 (GCT). The comparative analysis revealed that most of the tRNAs were structurally different, except for trnE (TTC), trnQ (TTG), and trnM (CAT). The structural features of tRNAs resulted ≥ 10 mismatched or wobble base pairings in 12 tRNAs, which reflects the nucleotide composition in both H- and L-strands. The mitogenome of N. nigricans also revealed two unique tandem repeats (ATTAT)8, and (TATTA)20 in the CR. Further, the conserved motif 5'-GACATA-3' and stable stem-loop structure was detected in the CRs of all Trionychidae species, which play an significant role in regulating transcription and replication in the mitochondrial genome. Further, the comparative analysis of Ka/Ks indicated negative selection in most of the protein coding genes (PCGs). The constructed Maximum Likelihood (ML) phylogeny using all PCGs showed clustering of N. nigricans with N. formosa. The resulting phylogeny illustrated the similar topology as described previously and consistent with the taxonomic classification. However, more sampling from different taxonomic groups of Testudines and studies on their mitogenomics are desirable for better understanding of the phylogenetic and evolutionary relationships.

Dataset Information

Complete mitochondrial genome and the phylogenetic position of the Burmese narrow-headed softshell turtle Chitra vandijki (Testudines: Trionychidae).

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