Ontology highlight
ABSTRACT:
SUBMITTER: Meyer JG
PROVIDER: S-EPMC8009190 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Meyer Jesse G JG Niemi Natalie M NM Pagliarini David J DJ Coon Joshua J JJ
Nature methods 20201123 12
Liquid chromatography-mass spectrometry (LC-MS) delivers sensitive peptide analysis for proteomics but requires extensive analysis time, reducing throughput. Here, we demonstrate that gas-phase peptide separation instead of LC enables fast proteome analysis. Using direct infusion-shotgun proteome analysis (DI-SPA) by data-independent acquisition mass spectrometry (DIA-MS), we demonstrate the targeted quantification of over 500 proteins within minutes of MS data collection (~3.5 proteins per seco ...[more]