Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice.

ABSTRACT: The understanding of the mechanisms underlying adipocyte differentiation and function has greatly benefited from the use of immortalized white preadipocyte cell lines. These cultured cell lines, however, have limitations. They do not fully capture the diverse functional spectrum of the heterogenous adipocyte populations that are now known to exist within white adipose depots. To provide a more physiologically relevant model to study the complexity of white adipose tissue, a protocol has been developed and optimized to enable simultaneous isolation of primary white and brown adipocyte progenitors from newborn mice, their rapid expansion in culture, and their differentiation in vitro into mature, fully functional adipocytes. The primary advantage of isolating primary cells from newborn, rath

SUBMITTER: Galmozzi A 

PROVIDER: S-EPMC8009622 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature