Inhibition of PKCε induces primordial germ cell reprogramming into pluripotency by HIF1&2 upregulation and histone acetylation.

ABSTRACT: Historically, primordial germ cells (PGCs) have been a good model to study pluripotency. Despite their low numbers and limited accessibility in the mouse embryo, they can be easily and rapidly reprogrammed at high efficiency with external physicochemical factors and do not require transcription factor transfection. Employing this model to deepen our understanding of cell reprogramming, we specifically aimed to determine the relevance of Ca²⁺ signal transduction pathway components in the reprogramming process. Our results showed that PGC reprogramming requires a normal extracellular [Ca²⁺] range, in contrast to neoplastic or transformed cells, which can continue to proliferate in Ca²⁺-deficient media, differentiating normal reprogramming from neoplastic tran

SUBMITTER: Moratilla A 

PROVIDER: S-EPMC8012778 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature