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5-Aminolevulinic Acid Improves Morphogenesis and Na⁺ Subcellular Distribution in the Apical Cells of Cucumis sativus L. Under Salinity Stress.

ABSTRACT: Soil salinity causes damage to plants and a reduction in output. A natural plant growth regulator, 5-aminolevulinic acid (ALA), has been shown to promote plant growth under abiotic stress conditions. In the present study, we assessed the effects of exogenously applied ALA (25 mg L^-1) on the root architecture and Na⁺ distribution of cucumber (Cucumis sativus L.) seedlings under moderate NaCl stress (50 mmol L^-1). The results showed that exogenous ALA improved root length, root volume, root surface area, and cell activity in the root tips, which were inhibited under salt stress. In addition, although salinity stress increased the subcellular Na⁺ contents, such as those of the cell wall, nucleus, plastid, and mitochondria, ALA treatment reduced these Na⁺ contents, except the soluble fraction. Molecular biological analysis revealed that ALA application upregulated both the SOS1 and HA3 transcriptional and translational levels, which suggested that the excretion of Na⁺ into the cytoplasm cloud was promoted by exogenous ALA. Meanwhile, exogenously applied ALA also upregulated the gene and protein expression of NHX1 and VHA-A under salinity stress, which suggested that the compartmentalization of Na⁺ to the vacuole was enhanced. Overall, exogenous ALA mitigated the damage caused by NaCl in cucumber by enhancing Na⁺ redistribution and increasing the cytoactivity of root cells.

SUBMITTER: Wu Y

PROVIDER: S-EPMC8012848 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Seeds germination and seedlings growth of Cucumis sativus and Solanum lycopersicum were monitored in in vitro and in vivo experiments after application of different concentrations of NaCl (25, 50, 100 and 200 mM). Photosynthetic pigments content and the biochemical responses of C. sativus and S. lycopersicum were assessed. Salinity stress slightly delayed the seeds germination rate and significantly reduced the percentage of germination as well as shoot length under the highest salt concentration (200 mM) in cucumber. Furthermore, root length was decreased significantly in all treatments. Whereas, in tomato, a prominent delay in seeds germination rate, the germination percentage and seedlings growth (shoot and root lengths) were significantly influenced under all concentrations of NaCl. Fresh and dry weights were reduced prominently in tomato compared to cucumber. Photosynthetic pigments content was reduced but with pronounced decreasing in tomato compared to cucumber. Secondary metabolites profiling in both plants under stress was varied from tomato to cucumber. The content of saponins, proline and total antioxidant capacity was reduced more prominently in tomato as compared to cucumber. On the other hand, the content of phenolics and flavonoids was increased in both plants with pronounced increase in tomato particularly under the highest level of salinity stress. The metabolomic profiling in stressful plants was significantly influenced by salinity stress and some bioactive secondary metabolites was enhanced in both cucumber and tomato plants. The enhancement of secondary metabolites under salinity stress may explain the tolerance and sensitivity of cucumber and tomato under salinity stress. The metabolomic evaluation combined with multivariate data analysis revealed a similar mechanism of action of plants to mediate stress, with variant level of this response in both plant species. Based on these results, the effect of salinity stress on seeds germination, seedlings growth and metabolomic content of plants was discussed in terms of tolerance and sensitivity of plants to salinity stress.

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5-Aminolevulinic Acid Improves Morphogenesis and Na⁺ Subcellular Distribution in the Apical Cells of Cucumis sativus L. Under Salinity Stress.

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5-Aminolevulinic Acid Improves Morphogenesis and Na+ Subcellular Distribution in the Apical Cells of Cucumis sativus L. Under Salinity Stress.

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OmicsDI is part of the ELIXIR infrastructure

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5-Aminolevulinic Acid Improves Morphogenesis and Na⁺ Subcellular Distribution in the Apical Cells of Cucumis sativus L. Under Salinity Stress.