Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro.

ABSTRACT:

Background information

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from São Paulo state (Brazil).

Results

Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space.

Conclusions and significance

This study contributes to a better understanding

SUBMITTER: Caldas LA 

PROVIDER: S-EPMC8013410 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature