3D-printed microplate inserts for long term high-resolution imaging of live brain organoids.

ABSTRACT:

Background

Organoids are a reliable model used in the study of human brain development and under pathological conditions. However, current methods for brain organoid culture generate tissues that range from 0.5 to 2 mm of size, which need to be constantly agitated to allow proper oxygenation. The culture conditions are, therefore, not suitable for whole-brain organoid live imaging, required to study developmental processes and disease progression within physiologically relevant time frames (i.e. days, weeks, months).

Results

Here we designed 3D-printed microplate inserts adaptable to standard 24 multi-well plates, which allow the growth of multiple organoids in pre-defined and fixed XYZ coordinates. This innovation facilitates high-resolution imaging of whole-cerebral organo

SUBMITTER: Oksdath Mansilla M 

PROVIDER: S-EPMC8015192 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature