Ontology highlight
ABSTRACT:
SUBMITTER: de Vries RMJM
PROVIDER: S-EPMC8017705 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
de Vries Rens M J M RMJM Meijer Femke A FA Doveston Richard G RG Leijten-van de Gevel Iris A IA Brunsveld Luc L
Proceedings of the National Academy of Sciences of the United States of America 20210201 6
Cooperative ligand binding is an important phenomenon in biological systems where ligand binding influences the binding of another ligand at an alternative site of the protein via an intramolecular network of interactions. The underlying mechanisms behind cooperative binding remain poorly understood, primarily due to the lack of structural data of these ternary complexes. Using time-resolved fluorescence resonance energy transfer (TR-FRET) studies, we show that cooperative ligand binding occurs ...[more]