Unknown

Dataset Information

0

Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc Min/+ Mice: Relation to Metabolism and Gut Microbiota Composition.


ABSTRACT: Free fatty acid receptor 2 (FFAR2) has been reported as a tumor suppressor in colon cancer development. The current study investigated the effects of FFAR2 signaling on energy metabolism and gut microbiota profiling in a colorectal cancer mouse model (Apc Min/+ ). Ffar2 deficiency promoted colonic polyp development and enhanced fatty acid oxidation and bile acid metabolism. Gut microbiome sequencing analysis showed distinct clustering among wild-type, Apc Min/+ , and Apc Min/+ -Ffar2 -/- mice. The relative abundance of Flavobacteriaceae and Verrucomicrobiaceae was significantly increased in the Apc Min/+ -Ffar2 -/- mice compared to the Apc Min/+ mice. In addition, knocking-down FFAR2 in the human colon cancer cell lines (SW480 and HT29) resulted in increased expression of several key enzymes in fatty acid oxidation, such as carnitine palmitoyltransferase 2, acyl-CoA dehydrogenase, long-chain acyl-CoA dehydrogenase, C-2 to C-3 short chain, and hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase, alpha subunit. Collectively, these results demonstrated that Ffar2 deficiency significantly altered profiles of fatty acid metabolites and gut microbiome, which might promote colorectal cancer development.

SUBMITTER: Huang YW 

PROVIDER: S-EPMC8020170 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6838415 | biostudies-literature
| S-EPMC9797476 | biostudies-literature
| S-EPMC6996958 | biostudies-literature
| S-EPMC5519705 | biostudies-literature
| S-EPMC6842652 | biostudies-literature
| S-EPMC2633613 | biostudies-literature
| S-EPMC5057043 | biostudies-literature
| S-EPMC8429936 | biostudies-literature
| S-EPMC5511216 | biostudies-literature
| S-EPMC8065027 | biostudies-literature