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Assessing of growth, antioxidant enzymes, and phytohormone regulation in Cucurbita pepo under cadmium stress.

ABSTRACT: One of the major problems worldwide is soil pollution by trace metal elements, which limits plant productivity and threatens human health. In this work, we have studied the effect of different concentrations of cadmium on Cucurbita pepo plants, evaluating different physiological and biochemical parameters: hormone signaling, metabolite concentration (malondialdehyde and hydrogen peroxide) and, in addition, the antioxidant enzyme activities of catalase and superoxide dismutase were evaluated. The production of biomass decreased under the Cd-stress. The results showed that C. pepo accumulates higher amounts of Cd²⁺ in roots than in shoots and fruits. Cd²⁺ differently affected the content of endogenous phytohormones. Furthermore, data suggest an essential involvement of roots in the regulation of tolerance to trace elements. As a result, indole acetic acid content increased in roots of treated plants, indicating that this phytohormone can stimulate root promotion and growth under Cd-stress. Similarly, salicylic acid content in roots and shoots increased in response to Cd²⁺, as well as abscisic acid levels in roots and fruits. In roots, the rambling accumulation pattern observed for jasmonic acid and salicylic acid suggests the lack of a specific regulation role against trace element toxicity. The activity of catalase and superoxide dismutase decreased, disrupted by the metal stress. However, the proline, malondialdehyde and hydrogen peroxide content significantly increased in Cd²⁺in all the analyzed tissues of the stressed plants. All these data suggest that C. pepo plants are equipped with an effective antioxidant mechanism against oxidative stress induced by cadmium up to a concentration of 500 μM.

SUBMITTER: Labidi O

PROVIDER: S-EPMC8020919 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background and aimsNectar is a very complex mixture of substances. Some components (sugars and amino acids) are considered primary alimentary rewards for animals and have been investigated and characterized in numerous species for many years. In contrast, nectar proteins have been the subject of few studies and little is known of their function. Only very recently have detailed studies and characterization of nectar proteins been undertaken, and then for only a very few species. This current work represents a first step in the identification of a protein profile for the floral nectar of Cucurbita pepo. In this regard, the species studied is of particular interest in that it is monoecious with unisexual flowers and, consequently, it is possible that nectar proteins derived from male and female flowers may differ.MethodsManually excised spots from two-dimensional (2-D) electrophoresis were subjected to in-gel protein digestion. The resulting peptides were sequenced using nanoscale LC-ESI/MS-MS (liquid chromatography-electrospray ionization/tandem mass spectrometry). An MS/MS ions search was carried out in Swiss-Prot and NCBInr databases using MASCOT software.Key resultsTwo-dimensional electrophoresis revealed a total of 24 spots and a different protein profile for male and female flower nectar. Four main proteins recognized by 2-D electrophoresis most closely resemble β-d-xylosidases from Arabidopsis thaliana and have some homology to a β-d-xylosidase from Medicago varia. They were present in similar quantities in male and female flowers and had the same molecular weight, but with slightly different isoelectric points.ConclusionsA putative function for xylosidases in floral nectar of C. pepo is proposed, namely that they may be involved in degrading the oligosaccharides released by the nectary cell walls in response to hydrolytic enzymes produced by invading micro-organisms. Several types of oligosaccharides have been reported to increase the pathogenic potential of micro-organisms. Thus, it is possible that such a mechanism may reduce the virulence of pathogens present in nectar.

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Assessing of growth, antioxidant enzymes, and phytohormone regulation in Cucurbita pepo under cadmium stress.

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