Insert L1 is a central hub for allosteric regulation of USP1 activity.
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ABSTRACT:
SUBMITTER: Dharadhar S
PROVIDER: S-EPMC8024992 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Publications
Insert L1 is a central hub for allosteric regulation of USP1 activity.
Dharadhar Shreya S van Dijk Willem J WJ Scheffers Serge S Fish Alexander A Sixma Titia K TK
EMBO reports 20210223 4
During DNA replication, the deubiquitinating enzyme USP1 limits the recruitment of translesion polymerases by removing ubiquitin marks from PCNA to allow specific regulation of the translesion synthesis (TLS) pathway. USP1 activity depends on an allosteric activator, UAF1, and this is tightly controlled. In comparison to paralogs USP12 and USP46, USP1 contains three defined inserts and lacks the second WDR20-mediated activation step. Here we show how inserts L1 and L3 together limit intrinsic US ...[more]
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