Perivascular macrophages create an intravascular niche for CD8⁺ T cell localisation prior to the onset of fatal experimental cerebral malaria.

ABSTRACT:

Objectives

The immunologic events that build up to the fatal neurological stage of experimental cerebral malaria (ECM) are incompletely understood. Here, we dissect immune cell behaviour occurring in the central nervous system (CNS) when Plasmodium berghei ANKA (PbA)-infected mice show only minor clinical signs.

Methods

A 2-photon intravital microscopy (2P-IVM) brain imaging model was used to study the spatiotemporal context of early immunological events in situ during ECM.

Results

Early in the disease course, antigen-specific CD8⁺ T cells came in contact and arrested on the endothelium of post-capillary venules. CD8⁺ T cells typically adhered adjacent to, or were in the near vicinity of, perivascular macrophages (PVMs) that line po

SUBMITTER: Qin J 

PROVIDER: S-EPMC8026342 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature