Imaging Tumor-Infiltrating Lymphocytes in Brain Tumors with [⁶⁴Cu]Cu-NOTA-anti-CD8 PET.

ABSTRACT:

Purpose

Glioblastoma (GBM) is the most common malignant brain tumor in adults. Various immunotherapeutic approaches to improve patient survival are being developed, but the molecular mechanisms of immunotherapy resistance are currently unknown. Here, we explored the ability of a humanized radiolabeled CD8-targeted minibody to noninvasively quantify tumor-infiltrating CD8-positive (CD8⁺) T cells using PET.

Experimental design

We generated a peripheral blood mononuclear cell (PBMC) humanized immune system (HIS) mouse model and quantified the absolute number of CD8⁺ T cells by flow cytometry relative to the [⁶⁴Cu]Cu-NOTA-anti-CD8 PET signal. To evaluate a patient-derived orthotopic GBM HIS model, we intracranially injected cells into NOG mice, h

SUBMITTER: Nagle VL 

PROVIDER: S-EPMC8026513 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature