Genome-wide identification of microRNAs regulating the human prion protein.

ABSTRACT: The cellular prion protein (PrP^C ) is best known for its misfolded disease-causing conformer, PrP^Sc . Because the availability of PrP^C is often limiting for prion propagation, understanding its regulation may point to possible therapeutic targets. We sought to determine to what extent the human microRNAome is involved in modulating PrP^C levels through direct or indirect pathways. We probed PrP^C protein levels in cells subjected to a genome-wide library encompassing 2019 miRNA mimics using a robust time-resolved fluorescence-resonance screening assay. Screening was performed in three human neuroectodermal cell lines: U-251 MG, CHP-212 and SH-SY5Y. The three screens yielded 17 overlapping high-confidence miRNA mimic hits, 13 of which wer

SUBMITTER: Pease D 

PROVIDER: S-EPMC8028613 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature