Browse
Submit Data
Databases
API
Help

Dataset Information

6 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA screening of Drosophila suzukii predators in berry field orchards shows new predatory taxonomical groups.

ABSTRACT: Drosophila suzukii (spotted wing drosophila, SWD) is a pandemic quarantine pest that attacks mostly red fruits. The high number of life cycles per year, its ability to rapidly invade and spread across new habitats, and highly polyphagous nature, makes this a particularly aggressive invasive species, for which efficient control methods are currently lacking. The use of native natural predators is particularly promising to anchor sustainable and efficient measures to control SWD. While several field studies have suggested the presence of potential predatory species in infested orchards, only a few confirmed the presence of SWD DNA in predators' gut content. Here, we use a DNA-based approach to identify SWD predators among the arthropod diversity in South Europe, by examining the gut content of potential predator specimens collected in SWD-infested berry fields in North Portugal. These specimens were morphologically identified to the family/order, and their gut content was screened for the presence of SWD DNA using PCR. New SWD predatory taxonomical groups were identified, as Opiliones and Hemerobiidae, in addition to known SWD predators, such as Hemerobiidae, Chrysopidae, Miridae, Carabidae, Formicidae and Araneae. Additionally, the presence of a spider family, Uloboridae, in the orchards was recorded for the first time, posing this family as another SWD-candidate predator. This study sets important bases to further investigate the potential large-scale use of some of these confirmed predator taxa for SWD control in South Europe.

SUBMITTER: Sario S

PROVIDER: S-EPMC8031375 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Population Dynamics of Drosophila suzukii in Coastal and Mainland Sweet Cherry Orchards of Greece.

Project description:Despite the recent invasion and wide spread of Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Europe, little is known regarding its population trends in coastal areas of the southern Mediterranean countries. Using adult trapping and fruit sampling, we studied the population dynamics of D. suzukii in coastal and mainland (semi-highland) cherry orchards of Greece, from 2018 to 2020. Adults were captured in traps baited with apple cider vinegar, placed in conventional and unmanaged sweet-cherry orchards, and in neighbouring wild growing hosts. Sampling of sweet-cherry fruit to assess infestation levels was conducted from early and late-ripening cherry cultivars in both areas. Adults were captured throughout the year in the coastal area with two peaks registered in spring and late-autumn. Captures were nearly zero during the hot summer months. Flight activity exhibited only one peak in autumn at the mainland area, and ceased during winter and spring. Captures in wild hosts were lower during the sweet-cherry ripening period than later in the season. Higher sweet-cherry infestation levels were recorded in the coastal than in the mainland area and in unmanaged than in commercial orchards. Both early and late-ripening cultivars were highly susceptible to D. suzukii infestation in the coastal area. Infestation rates were higher in late-ripening cultivars than in early-ripening ones in the mainland area. We conclude that D. suzukii has well adapted to the Mediterranean climate of Greece, and is able to progressively exploit several crops and wild hosts of mainland and coastal areas.

| S-EPMC7564280 | biostudies-literature

Deterrent Effects of Essential Oils on Spotted-Wing Drosophila (Drosophila suzukii): Implications for Organic Management in Berry Crops.

Project description:Due to concerns about frequent applications of spinosad and other broad spectrum insecticides for managing spotted-wing drosophila (Drosophila suzukii Matsumura, SWD), we investigated the use of essential oils as an alternative to current insecticides. Essential oils from a number of plant species have been studied for their attraction and deterrence of SWD. However, these botanical products have not been thoroughly tested in the field. We conducted laboratory and field studies to determine the efficacy of botanical products, including lavender (Lavandula angustifolia Mill.) oil, catnip (Nepeta cataria L.) oil, KeyPlex Ecotrol® PLUS, and KeyPlex Sporan® EC2 on preventing SWD infestation in raspberry (Rubus idaeus L.) and blueberry (Vacciniumcorymbosum L.) crops. In a two-choice laboratory bioassay, lavender oil, Ecotrol, and Sporan treatments deterred SWD from a yeast-cornmeal-sugar based fly diet. In the field trials, raspberry fruit treated with Ecotrol had lower SWD infestation (6%), compared to the control (17%), and was comparable to spinosad (6%). No differences were seen in blueberry infestation. The combination of essential oils in Ecotrol may work to decrease SWD fruit infestation under certain conditions in the field, however more research is needed on the longevity of these products.

| S-EPMC7469169 | biostudies-literature

Diel periodicity of Drosophila suzukii (Diptera: Drosophilidae) under field conditions.

Project description:Drosophila suzukii Matsumura (Diptera: Drosophilidae), an economically important pest of blueberry and other thin-skinned fruits, persists and prolifically reproduces under seemingly lethal climatic conditions in the field. However, behavioral and physiological mechanisms employed by D. suzukii to tolerate such extreme climatic conditions in the field are unknown. The primary objective of this project was to investigate diel periodicity of D. suzukii and their reproductive success under field conditions as related by climatic factors such as temperature and relative humidity. Results show that D. suzukii reproductive success was significantly higher during the night (including dawn and dusk periods) than the day in terms of oviposition, pupation, adult eclosion, and the number of progeny per female. Female D. suzukii reproductive success was not significantly different between specific regions of a blueberry bush in relation to the amount of shade provided by the canopy. Our studies indicate that D. suzukii flight activity is crepuscular and is sensitive to fluctuations in temperature and relative humidity. Results also suggest that the majority of fly activity during peak hours is concentrated in areas around the border and within the center of blueberry orchards with little activity in the surrounding wooded areas. These findings suggest that D. suzukii prefers microclimate with mild temperatures and high humidity, and does not function well when exposed to direct sunlight with extreme heat. The authors propose that D. suzukii management strategies should be implemented during the early morning and immediately before darkness to maximize efficacy.

| S-EPMC5302832 | biostudies-literature

Degree day models to forecast the seasonal phenology of Drosophila suzukii in tart cherry orchards in the Midwest U.S.

Project description:Spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is an invasive economic pest of soft-skinned and stone fruit across the globe. Our study establishes both a predictive generalized linear mixed model (GLMM), and a generalized additive mixed model (GAMM) of the dynamic seasonal phenology of D. suzukii based on four years of adult monitoring trap data in Wisconsin tart cherry orchards collected throughout the growing season. The models incorporate year, field site, relative humidity, and degree days (DD); and relate these factors to trap catch. The GLMM estimated a coefficient of 2.21 for DD/1000, meaning for every increment of 1000 DD, trap catch increases by roughly 9 flies. The GAMM generated a curve based on a cubic regression smoothing function of DD which approximates critical DD points of first adult D. suzukii detection at 1276 DD, above average field populations beginning at 2019 DD, and peak activity at 3180 DD. By incorporating four years of comprehensive seasonal phenology data from the same locations, we introduce robust models capable of using DD to forecast changing adult D. suzukii populations in the field leading to the application of more timely and effective management strategies.

| S-EPMC7182266 | biostudies-literature

Effects of Variety and Grape Berry Condition of Vitis vinifera on Preference Behavior and Performance of Drosophila suzukii.

Project description:Drosophila suzukii is an invasive fruit pest and represents a potential economic threat to viticulture. After first observations of D. suzukii in Europe in 2008, research mainly focused on the evaluation of the host range and infestation risk for fruit and berry crops. However, the risk assessment of D. suzukii in viticulture has only recently started. Understanding the factors influencing preferences of D. suzukii for host species and varieties as well as offspring performance is essential to improve management strategies. We investigated the field infestation of different grape varieties across Baden-Wuerttemberg, southwestern Germany, between 2015 and 2018. Moreover, we performed dual-choice assays in the laboratory to investigate whether adults show preferences for certain varieties and whether offspring performance differs between varieties. Furthermore, we studied the impact of grape damage on choice behavior. Field monitoring revealed that D. suzukii show preferences for red varieties, whereas almost no oviposition occurred in white varieties. The results of dual-choice assays confirmed that D. suzukii preference and performance are influenced by grape variety and that flies preferred damaged over intact "Pinot Noir", "Pinot Blanc", and "Müller-Thurgau" berries. Overall, these findings may have important implications for winegrowers regarding cultivated varieties, grape health, and insecticide reduction.

| S-EPMC6955987 | biostudies-literature

Detection and monitoring of Drosophila suzukii in raspberry and cherry orchards with volatile organic compounds in the USA and Europe.

Project description:Spotted wing drosophila (SWD) causes significant economic loss in fruit crops to growers worldwide. There is immediate need for efficacious and selective monitoring tools that can detect infestations early. Previously, volatile organic compounds derived from apple were studied and a quinary chemical component blend (QB) was identified as the key SWD attractant in a blueberry orchard in the United States. This study's aim was to determine whether previously observed QB efficacy, selectivity, and early detection levels could be attained within raspberry and cherry fields in the USA and Europe. Results demonstrated that sticky trap baited QB dispenser provided earlier SWD detection potential than the usually adopted apple cider vinegar (ACV) trap. The number of SWD captured/trap by QB baited trapping systems was significantly lower than that of the ACV trap. However, percent SWD/trap of QB baited traps was same within cherry. Lower non-target capture will save farmer/grower's labor and time allocated to traps installation and drosophila species identification. Within the USA, SWD selectivity of QB baited liquid traps was consistently greater than sticky trap in raspberry field, suggesting that the QB dispenser can be an alternative to the standard ACV lure and that trap design could improve selectivity further.

| S-EPMC7994672 | biostudies-literature