Browse
Submit Data
Databases
API
Help

Dataset Information

18 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Estimating the flexural rigidity of Arabidopsis inflorescence stems: Free-vibration test vs. three-point bending test.

ABSTRACT: The mechanical strength of a plant stem (a load-bearing organ) helps the plant resist drooping, buckling and fracturing. We previously proposed a method for quickly evaluating the stiffness of an inflorescence stem in the model plant Arabidopsis thaliana based on measuring its natural frequency in a free-vibration test. However, the relationship between the stiffness and flexural rigidity of inflorescence stems was unclear. Here, we compared our previously described free-vibration test with the three-point bending test, the most popular method for calculating the flexural rigidity of A. thaliana stems, and examined the extent to which the results were correlated. Finally, to expand the application range, we present an example of a modified free-vibration test. Our results provide a reference for improving estimates of the flexural rigidity of A. thaliana inflorescence stems.

SUBMITTER: Nakata MT

PROVIDER: S-EPMC8034677 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

<i>Arabidopsis</i> Myosins XI1, XI2, and XIK Are Crucial for Gravity-Induced Bending of Inflorescence Stems.

Project description:Myosins and actin filaments in the actomyosin system act in concert in regulating cell structure and dynamics and are also assumed to contribute to plant gravitropic response. To investigate the role of the actomyosin system in the inflorescence stem gravitropism, we used single and multiple mutants affecting each of the 17 Arabidopsis myosins of class VIII and XI. We show that class XI but not class VIII myosins are required for stem gravitropism. Simultaneous loss of function of myosins XI1, XI2, and XIK leads to impaired gravitropic bending that is correlated with altered growth, stiffness, and insufficient sedimentation of gravity sensing amyloplasts in stem endodermal cells. The gravitropic defect of the corresponding triple mutant xi1 xi2 xik could be rescued by stable expression of the functional XIK:YFP in the mutant background, indicating a role of class XI myosins in this process. Altogether, our results emphasize the critical contributions of myosins XI in stem gravitropism of Arabidopsis.

| S-EPMC5174092 | biostudies-literature

Arabidopsis inflorescence stems: wild type vs sgr1

Project description:Transcriptional profiling of wild type and sgr1 of Arabidopsis inflorescence stems. Candidate genes which were down-regulated in sgr1 relative to wild type were identified.

2017-07-13 | GSE101296 | GEO

Arabidopsis inflorescence stems: wild type vs eal1

Project description:Transcriptional profiling of wild type and eal1 of Arabidopsis inflorescence stems. Candidate genes which were down-regulated in eal1 relative to wild type were identified.

2017-07-13 | GSE101293 | GEO

Flexural vibration systems with gyroscopic spinners.

Project description:In this paper, we study the spectral properties of a finite system of flexural elements connected by gyroscopic spinners. We determine how the eigenfrequencies and eigenmodes of the system depend on the gyricity of the spinners. In addition, we present a transient numerical simulation that shows how a gyroscopic spinner attached to the end of a hinged beam can be used as a 'stabilizer', reducing the displacements of the beam. We also discuss the dispersive properties of an infinite periodic system of beams with gyroscopic spinners at the junctions. In particular, we investigate how the band-gaps of the structure can be tuned by varying the gyricity of the spinners. This article is part of the theme issue 'Modelling of dynamic phenomena and localization in structured media (part 1)'.

| S-EPMC6732376 | biostudies-literature

Modeling the flexural rigidity of rod photoreceptors.

Project description:In vertebrate eyes, the rod photoreceptor has a modified cilium with an extended cylindrical structure specialized for phototransduction called the outer segment (OS). The OS has numerous stacked membrane disks and can bend or break when subjected to mechanical forces. The OS exhibits axial structural variation, with extended bands composed of a few hundred membrane disks whose thickness is diurnally modulated. Using high-resolution confocal microscopy, we have observed OS flexing and disruption in live transgenic Xenopus rods. Based on the experimental observations, we introduce a coarse-grained model of OS mechanical rigidity using elasticity theory, representing the axial OS banding explicitly via a spring-bead model. We calculate a bending stiffness of ?10(5) nN??m2, which is seven orders-of-magnitude larger than that of typical cilia and flagella. This bending stiffness has a quadratic relation to OS radius, so that thinner OS have lower fragility. Furthermore, we find that increasing the spatial frequency of axial OS banding decreases OS rigidity, reducing its fragility. Moreover, the model predicts a tendency for OS to break in bands with higher spring number density, analogous to the experimental observation that transgenic rods tended to break preferentially in bands of high fluorescence. We discuss how pathological alterations of disk membrane properties by mutant proteins may lead to increased OS rigidity and thus increased breakage, ultimately contributing to retinal degeneration.

| S-EPMC3552261 | biostudies-literature

Arabidopsis inflorescence stems: wild type vs eal1 and sgr1

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-07-13 | GSE101298 | GEO

Sequence dependence of DNA bending rigidity.

Project description:For many aspects of DNA-protein interaction, it is vital to know how DNA bending rigidity (or persistence length, a) depends on its sequence. We addressed this problem using the method based on cyclization of short DNA fragments, which allows very accurate determination of a. Our approach was based on assigning specific values of a to each of 10 distinct dinucleotide steps. We prepared DNA fragments, each about 200 bp in length, with various quasi-periodic sequences, measured their cyclization efficiencies (j factors), and fitted the data by the theoretical equation to obtain the values of a for each fragment. From these data, we obtained a set of a for the dinucleotide steps. To test this set, we used it to design DNA sequences that should correspond to very low and very high values of a, prepared the corresponding fragments, and determined their values of a experimentally. The measured and calculated values of a were very close to one another, confirming that we have found the correct solution to this long-standing problem. The same experimental data also allowed us to determine the sequence dependence of DNA helical repeat.

| S-EPMC2932579 | biostudies-literature

Flexural bending of southern Tibet in a retro foreland setting.

Project description:The highest elevation of the Tibetan Plateau, lying 5,700 m above sea level, occurs within the part of the Lhasa block immediately north of the India-Tibet suture zone (Yarlung Zangbo suture zone, YZSZ), being 700 m higher than the maximum elevation of more northern parts of the plateau. Various mechanisms have been proposed to explain this differentially higher topography and the rock uplift that led to it, invoking crustal compression or extension. Here we present the results of structural investigations along the length of the high elevation belt and suture zone, which rather indicate flexural bending of the southern margin of the Lhasa block (Gangdese magmatic belt) and occurrence of an adjacent foreland basin (Kailas Basin), both elements resulting from supra-crustal loading of the Lhasa block by the Zangbo Complex (Indian plate rocks) via the Great Counter Thrust. Hence we interpret the differential elevation of the southern margin of the plateau as due originally to uplift of a forebulge in a retro foreland setting modified by subsequent processes. Identification of this flexural deformation has implications for early evolution of the India-Tibet continental collision zone, implying an initial (Late Oligocene) symmetrical architecture that subsequently transitioned into the present asymmetrical wedge architecture.

| S-EPMC4502525 | biostudies-literature

Arabidopsis inflorescence stems: wild type vs eal1 and sgr1

Project description:Arabidopsis inflorescence stems: wild type vs eal1 and sgr1

| PRJNA393967 | ENA

Flexural bending to approximate cortical forces exerted by electrocorticography (ECoG) arrays.

Project description:Objective.The force that an electrocorticography (ECoG) array exerts on the brain manifests when it bends to match the curvature of the skull and cerebral cortex. This force can negatively impact both short-term and long-term patient outcomes. Here we provide a mechanical characterization of a novel liquid crystal polymer (LCP) ECoG array prototype to demonstrate that its thinner geometry reduces the force potentially applied to the cortex of the brain.Approach.We built a low-force flexural testing machine to measure ECoG array bending forces, calculate their effective flexural moduli, and approximate the maximum force they could exerted on the human brain.Main results.The LCP ECoG prototype was found to have a maximal force less than 20% that of any commercially available ECoG arrays that were tested. However, as a material, LCP was measured to be as much as 24× more rigid than silicone, which is traditionally used in ECoG arrays. This suggests that the lower maximal force resulted from the prototype's thinner profile (2.9×-3.25×).Significance.While decreasing material stiffness can lower the force an ECoG array exhibits, our LCP ECoG array prototype demonstrated that flexible circuit manufacturing techniques can also lower these forces by decreasing ECoG array thickness. Flexural tests of ECoG arrays are necessary to accurately assess these forces, as material properties for polymers and laminates are often scale dependent. As the polymers used are anisotropic, elastic modulus cannot be used to predict ECoG flexural behavior. Accounting for these factors, we used our four-point flexure testing procedure to quantify the forces exerted on the brain by ECoG array bending. With this experimental method, ECoG arrays can be designed to minimize force exerted on the brain, potentially improving both acute and chronic clinical utility.

| S-EPMC10002477 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data