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Use of Chou's 5-steps rule to predict the subcellular localization of gram-negative and gram-positive bacterial proteins by multi-label learning based on gene ontology annotation and profile alignment.

ABSTRACT: To date, many proteins generated by large-scale genome sequencing projects are still uncharacterized and subject to intensive investigations by both experimental and computational means. Knowledge of protein subcellular localization (SCL) is of key importance for protein function elucidation. However, it remains a challenging task, especially for multiple sites proteins known to shuttle between cell compartments to perform their proper biological functions and proteins which do not have significant homology to proteins of known subcellular locations. Due to their low-cost and reasonable accuracy, machine learning-based methods have gained much attention in this context with the availability of a plethora of biological databases and annotated proteins for analysis and benchmarking. Various predictive models have been proposed to tackle the SCL problem, using different protein sequence features pertaining to the subcellular localization, however, the overwhelming majority of them focuses on single localization and cover very limited cellular locations. The prediction was basically established on sorting signals, amino acids compositions, and homology. To improve the prediction quality, focus is actually on knowledge information extracted from annotation databases, such as protein-protein interactions and Gene Ontology (GO) functional domains annotation which has been recently a widely adopted and essential information for learning systems. To deal with such problem, in the present study, we considered SCL prediction task as a multi-label learning problem and tried to label both single site and multiple sites unannotated bacterial protein sequences by mining proteins homology relationships using both GO terms of protein homologs and PSI-BLAST profiles. The experiments using 5-fold cross-validation tests on the benchmark datasets showed a significant improvement on the results obtained by the proposed consensus multi-label prediction model which discriminates six compartments for Gram-negative and five compartments for Gram-positive bacterial proteins.

SUBMITTER: Bouziane H

PROVIDER: S-EPMC8035964 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND: Although many computational methods have been developed to predict protein subcellular localization, most of the methods are limited to the prediction of single-location proteins. Multi-location proteins are either not considered or assumed not existing. However, proteins with multiple locations are particularly interesting because they may have special biological functions, which are essential to both basic research and drug discovery. RESULTS: This paper proposes an efficient multi-label predictor, namely mGOASVM, for predicting the subcellular localization of multi-location proteins. Given a protein, the accession numbers of its homologs are obtained via BLAST search. Then, the original accession number and the homologous accession numbers of the protein are used as keys to search against the Gene Ontology (GO) annotation database to obtain a set of GO terms. Given a set of training proteins, a set of T relevant GO terms is obtained by finding all of the GO terms in the GO annotation database that are relevant to the training proteins. These relevant GO terms then form the basis of a T-dimensional Euclidean space on which the GO vectors lie. A support vector machine (SVM) classifier with a new decision scheme is proposed to classify the multi-label GO vectors. The mGOASVM predictor has the following advantages: (1) it uses the frequency of occurrences of GO terms for feature representation; (2) it selects the relevant GO subspace which can substantially speed up the prediction without compromising performance; and (3) it adopts an efficient multi-label SVM classifier which significantly outperforms other predictors. Briefly, on two recently published virus and plant datasets, mGOASVM achieves an actual accuracy of 88.9% and 87.4%, respectively, which are significantly higher than those achieved by the state-of-the-art predictors such as iLoc-Virus (74.8%) and iLoc-Plant (68.1%). CONCLUSIONS: mGOASVM can efficiently predict the subcellular locations of multi-label proteins. The mGOASVM predictor is available online at http://bioinfo.eie.polyu.edu.hk/mGoaSvmServer/mGOASVM.html.

Dataset Information

Use of Chou's 5-steps rule to predict the subcellular localization of gram-negative and gram-positive bacterial proteins by multi-label learning based on gene ontology annotation and profile alignment.

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