Molecularly Imprinted Polymer-Based Sensors for Priority Pollutants.
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ABSTRACT:
SUBMITTER: Zarejousheghani M
PROVIDER: S-EPMC8037679 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Over the last decades, molecularly imprinted polymers (MIPs) have emerged as selective synthetic receptors that have a selective binding site for specific analytes/target molecules. MIPs are synthetic analogues to the natural biological antigen-antibody system. Owing to the advantages they exhibit, such as high stability, simple synthetic procedure, and cost-effectiveness, MIPs have been widely used as receptors/sensors for the detection and monitoring of a variety of analytes. Moreover, integrating electrochemical sensors with MIPs offers a promising approach and demonstrates greater potential over traditional MIPs. In this review, we have compiled the methods and techniques for the production of MIP-based electrochemical sensors along with the applications of reported MIP sensors for a variety of analytes. A comprehensive in-depth analysis of recent trends reported on picomolar (pM/10-12 M)) and beyond picomolar concentration LOD (≥pM) achieved using MIPs sensors is reported. Finally, we discuss the challenges faced and put forward future perspectives along with our conclusion.
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Project description:A versatile approach for the preparation of photoswitchable molecularly imprinted polymers (MIPs) is proposed where the selective recognition and the photoresponsive function are assumed by two different monomers. As a proof of concept, MIP microspheres were synthesized by precipitation polymerization for recognizing terbutylazine, a triazine-type herbicide. Formation of the selective binding sites was based upon H-bonding interactions between the template and the functional monomer methacrylic acid, whereas a polymerizable spiropyran unit was incorporated into the polymer matrix to provide light-controllable characteristics. A trifunctional monomer, trimethylolpropane trimethacrylate, was used as a cross-linker. The imprinted particles exhibited considerable morphological differences compared to their nonimprinted counterparts as observed by scanning electron microscopy. The imprinting effect was confirmed by equilibrium rebinding studies. The photoresponsiveness of the polymer particles was visualized by fluorescence microscopy and further characterized by spectroscopy. The template binding behavior could be regulated by alternating UV and visible light illumination when analyte release and uptake was observed, respectively. Binding isotherms fitted by the Freundlich model revealed the photomodulation of the number of binding sites and their average affinity. This facile synthetic approach may give an attractive starting point to endow currently existing highly selective MIPs with photoswitchable properties, thereby extending the scope of spiropyran-based photoresponsive smart materials.
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Project description:Alzheimer's disease (AD) is one of the most common forms of dementia affecting millions of people worldwide. Currently, an easy and effective form of diagnosis is missing, which significantly hinders a possible improvement of the patient's quality of life. In this context, biosensors emerge as a future solution, opening the doors for preventive medicine and allowing the premature diagnosis of numerous pathologies. This work presents a pioneering biosensor that combines a bottom-up design approach using paper as a platform for the electrochemical recognition of peptide amyloid ?-42 (A?-42), a biomarker for AD present in blood, associated with visible differences in the brain tissue and responsible for the formation of senile plaques. The sensor layer relies on a molecularly imprinted polymer as a biorecognition element, created on the carbon ink electrode's surface by electropolymerizing a mixture of the target analyte (A?-42) and a monomer (O-phenylenediamine) at neutral pH 7.2. Next, the template molecule was removed from the polymeric network by enzymatic and acidic treatments. The vacant sites so obtained preserved the shape of the imprinted protein and were able to rebind the target analyte. Morphological and chemical analyses were performed in order to control the surface modification of the materials. The analytical performance of the biosensor was evaluated by an electroanalytical technique, namely, square wave voltammetry. For this purpose, the analytical response of the biosensor was tested with standard solutions ranging from 0.1 ng/mL to 1 ?g/mL of A?-42. The linear response of the biosensor went down to 0.1 ng/mL. Overall, the developed biosensor offered numerous benefits, such as simplicity, low cost, reproducibility, fast response, and repeatability less than 10%. All together, these features may have a strong impact in the early detection of AD.
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Project description:In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro?polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.
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Project description:Rapid distribution of viral-induced diseases and weaknesses of common diagnostic platforms for accurate and sensitive identification of infected people raises an urgent demand for the design and fabrication of biosensors capable of early detection of viral biomarkers with high specificity. Accordingly, molecularly imprinted polymers (MIPs) as artificial antibodies prove to be an ideal preliminary detection platform for specific identification of target templates, with superior sensitivity and detection limit (DL). MIPs detect the target template with the "lock and key" mechanism, the same as natural monoclonal antibodies, and present ideal stability at ambient temperature, which improves their practicality for real applications. Herein, a 2D MIP platform consisting of decorated graphene oxide with the interconnected complex of polypyrrole-boronic acid is developed that can detect the trace of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen in aquatic biological samples with ultrahigh sensitivity/specificity with DL of 0.326 and 11.32 fg mL-1 using voltammetric and amperometric assays, respectively. Additionally, the developed MIP shows remarkable stability, selectivity, and accuracy toward detecting the target template, which paves the way for developing ultraspecific and prompt screening diagnostic configurations capable of detecting the antigen in 1 min or 20 s using voltammetric or amperometric techniques.
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Project description:The outbreak of COVID-19 has taken a large number of lives since 2019 and the death toll continues to increase all over the world. Recent data reports that about 27 lacs of people are infected with this virus till date and around 2 lacs are dead due to this pandemic. The situation in India is no way better. In India, almost all the states have become victim of this deadly pandemic. Considering the enormous population in India, citizens here are facing acute shortage of detection kits and many are dying even before the knowledge of their infection. The present treatise proposes a molecularly imprinted polymer (MIP) based technique for simple and rapid detection of COVID-19. The technique will be inexpensive, selective, reusable and easy to handle. It has been already implemented in our laboratory in order to detect the taste contributing agents found in tea. This article discusses the detailed methodology and the resultant analytical characteristic of the sensors developed so far and also outlines the suitability of the MIP technique towards fabrication of testing kits for rapid detection of COVID-19.
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Project description:Molecularly imprinted polymers (MIP) combine the selectivity of immunoaffinity chromatography with the robustness of common solid-phase extraction in what is referred to as molecularly imprinted solid-phase extraction (MISPE). This contribution shows how MIP design may be guided by pharmacophore modeling for the example of citrinin, which is an emerging mycotoxin from cereals. The obtained pharmacophore model allowed searching public databases for a set of citrinin-mimicking molecular surrogates. Imprinted and non-imprinted polymers were subsequently obtained through bulk and core-shell polymerization in the presence of these surrogates. Evaluation of their binding ability for citrinin and structurally related ochratoxin A revealed a promising MIP derived from rhodizonic acid. A protocol for MISPE of citrinin from cereals was subsequently developed and compared to immunoaffinity chromatography with respect to clean-up efficiency and recovery.
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Project description:The persistent use of pesticides in the agriculture field remains a serious issue related to public health. In the present work, molecularly imprinted polymer thin films were developed using electropolymerization of pyrrole (py) onto gold microelectrodes followed by electrodeposition for the selective detection of chlorpyrifos (CPF). The molecularly imprinted polymer (MIP) was synthesized by the electrochemical deposition method, which allowed in-line transfer of MIP on gold microelectrodes without using any additional adhering agents. Various parameters such as pH, monomer ratio, scan rate, and deposition cycle were optimized for sensor fabrication. The sensor was characterized at every stage of fabrication using various spectroscopic, microscopic, and electrochemical techniques. The sensor requires only 2 μL of the analyte and its linear detection range was found to be 1 μM to 1 fM. The developed sensor's limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.93 and 2.82 fM, respectively, with a sensitivity of 3.98 (μA/(μM)/ mm2. The sensor's shelf life was tested for 70 days. The applicability of the sensor in detecting CPF in fruit and vegetable samples was also assessed out with recovery % between 91 and 97% (RSD < 5%). The developed sensor possesses a huge commercial potential for on-field monitoring of pesticides.
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Project description:Trimethylamine N-oxide (TMAO), a microbiota-derived metabolite has been implicated in human health and disease. Its early detection in body fluids has been presumed to be significant in understanding the pathogenesis and treatment of many diseases. Hence, the development of reliable and rapid technologies for TMAO detection may augment our understanding of pathogenesis and diagnosis of diseases that TMAO has implicated. The present work is the first report on the development of a molecularly imprinted polymer (MIP) based electrochemical sensor for sensitive and selective detection of TMAO in body fluids. The MIP developed was based on the polypyrrole (PPy), which was synthesized via chemical oxidation polymerization method, with and without the presence of TMAO. The MIP, NIP and the non-sonicated polymer (PPy-TMAO) were separately deposited electrophoretically onto the hydrolyzed indium tin oxide (ITO) coated glasses. The chemical, morphological, and electrochemical behavior of MIP, non-imprinted polymer (NIP), and PPy-TMAO were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and electrochemical techniques. The detection response was recorded using differential pulse voltammetry (DPV), which revealed a decrease in the peak current with the increase in concentration of TMAO. The MIP sensor showed a dynamic detection range of 1-15 ppm with a sensitivity of 2.47 µA mL ppm-1 cm-2. The developed sensor is easy to construct and operate and is also highly selective to detect TMAO in body fluids such as urine. The present research provides a basis for innovative strategies to develop sensors based on MIP to detect other metabolites derived from gut microbiota that are implicated in human health and diseases.
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