Ontology highlight
ABSTRACT:
SUBMITTER: Liu N
PROVIDER: S-EPMC8038971 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Nature genetics 20210301 4
BCL11A, the major regulator of fetal hemoglobin (HbF, α<sub>2</sub>γ<sub>2</sub>) level, represses γ-globin expression through direct promoter binding in adult erythroid cells in a switch to adult hemoglobin (HbA, α<sub>2</sub>β<sub>2</sub>). To uncover how BCL11A initiates repression, we used CRISPR-Cas9, dCas9, dCas9-KRAB and dCas9-VP64 screens to dissect the γ-globin promoters and identified an activator element near the BCL11A-binding site. Using CUT&RUN and base editing, we demonstrate that ...[more]