High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.

ABSTRACT:

Objectives

The study aimed at identifying salivary microbiota in caries-free Chinese preschool children using high-throughput sequencing.

Methods

Saliva samples were obtained from 35 caries-free preschool children (18 boys and 17 girls) with primary dentition, and 16S ribosomal DNA (rDNA) V3-V4 hypervariable regions of the microorganisms were analyzed using Illumina MiSeq.

Results

At 97% similarity level, all of these reads were clustered into 334 operational taxonomic units (OTUs). Among these, five phyla (Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Candidate division TM7) and 13 genera (Streptococcus, Rothia, Granulicatella, Prevotella, Enterobacter, Veillonella, Neisseria, Staphylococcus,

SUBMITTER: Xu L 

PROVIDER: S-EPMC8042525 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature