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Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography.

ABSTRACT: RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the method and present two approaches: one that includes RNA isolation for maximum sensitivity and one that is suitable for partial automatization and is therefore less time-consuming. For complete details on the use and execution of this protocol, please refer to Ebbing et al. (2018).

SUBMITTER: Schild ES

PROVIDER: S-EPMC8044689 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Spatial transcriptomics of the nematode <i>Caenorhabditis elegans</i> using RNA tomography.

Schild Erik S ES Mars Jonas J Ebbing Annabel A Vivié Judith J Betist Marco M Korswagen Hendrik C HC

STAR protocols 20210330 2

RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode <i>Caenorhabditis elegans</i> to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the method and present two approaches: one that includes RNA isolation for maximum sensitivity and one that is suitable for partial automatization and is therefore less time-consuming. For complete ...[more]

PMID: 33870220

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