Targeted Phage Display-based Pulmonary Vaccination in Mice and Non-human Primates.

ABSTRACT:

Background

The extensive alveolar capillary network of the lungs is an attractive route for administration of several agents. One key functional attribute is the rapid onset of systemic action due to the absence of first-pass metabolism.

Methods

Here we applied a combinatorial approach for ligand-directed pulmonary delivery as a unique route for systemic targeting in vaccination.

Findings

We screened a phage display random peptide library in vivo to select, identify, and validate a ligand (CAKSMGDIVC) that specifically targets and is internalized through its receptor, α3β1 integrin, on the surface of cells lining the lung airways and alveoli and mediates CAKSMGDIVC-displaying phage binding and systemic delivery without compromising lung homeostasis. As a proof-

SUBMITTER: Staquicini DI 

PROVIDER: S-EPMC8049167 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature