Tools for experimental and computational analyses of off-target editing by programmable nucleases.

ABSTRACT: Genome editing using programmable nucleases is revolutionizing life science and medicine. Off-target editing by these nucleases remains a considerable concern, especially in therapeutic applications. Here we review tools developed for identifying potential off-target editing sites and compare the ability of these tools to properly analyze off-target effects. Recent advances in both in silico and experimental tools for off-target analysis have generated remarkably concordant results for sites with high off-target editing activity. However, no single tool is able to accurately predict low-frequency off-target editing, presenting a bottleneck in therapeutic genome editing, because even a small number of cells with off-target editing can be detrimental. Therefore, we recommend that at least on

SUBMITTER: Bao XR 

PROVIDER: S-EPMC8049448 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature