Unknown

Dataset Information

0

Simple methodology to visualize whole-brain microvasculature in three dimensions.


ABSTRACT: Significance: To explore brain architecture and pathology, a consistent and reliable methodology to visualize the three-dimensional cerebral microvasculature is beneficial. Perfusion-based vascular labeling is quick and easily deliverable. However, the quality of vascular labeling can vary with perfusion-based labels due to aggregate formation, leakage, rapid photobleaching, and incomplete perfusion. Aim: We describe a simple, two-day protocol combining perfusion-based labeling with a two-day clearing step that facilitates whole-brain, three-dimensional microvascular imaging and characterization. Approach: The combination of retro-orbital injection of Lectin-Dylight-649 to label the vasculature, the clearing process of a modified iDISCO+ protocol, and light-sheet imaging collectively enables a comprehensive view of the cerebrovasculature. Results: We observed ∼threefold increase in contrast-to-background ratio of Lectin-Dylight-649 vascular labeling over endogenous green fluorescent protein fluorescence from a transgenic mouse model. With light-sheet microscopy, we demonstrate sharp visualization of cerebral microvasculature throughout the intact mouse brain. Conclusions: Our tissue preparation protocol requires fairly routine processing steps and is compatible with multiple types of optical microscopy.

SUBMITTER: Khouri K 

PROVIDER: S-EPMC8056070 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5776138 | biostudies-literature
| S-EPMC3553090 | biostudies-literature
| S-EPMC3925763 | biostudies-literature
| S-EPMC4015294 | biostudies-literature
| S-EPMC5053282 | biostudies-literature
2013-07-24 | GSE37275 | GEO
| S-EPMC4987940 | biostudies-literature
| S-EPMC3985742 | biostudies-other
| S-EPMC9233036 | biostudies-literature
| S-EPMC5156535 | biostudies-literature