Rapid Determination of Antibody-Antigen Affinity by Mass Photometry.

ABSTRACT: Measurements of the specificity and affinity of antigen-antibody interactions are critically important for medical and research applications. In this protocol, we describe the implementation of a new single-molecule technique, mass photometry (MP), for this purpose. MP is a label- and immobilization-free technique that detects and quantifies molecular masses and populations of antibodies and antigen-antibody complexes on a single-molecule level. MP analyzes the antigen-antibody sample within minutes, allowing for the precise determination of the binding affinity and simultaneously providing information on the stoichiometry and the oligomeric state of the proteins. This is a simple and straightforward technique that requires only picomole quantities of protein and no expensive consumables.

SUBMITTER: Wu D 

PROVIDER: S-EPMC8056393 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature